Drosophila PSI controls circadian period and the phase of circadian behavior under temperature cycle via tim splicing
| Autor: | Naveh Evantal, Patrick Emery, Radhika S. Joshi, Sebastian Kadener, Jinli Ling, Lauren E. Foley |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
QH301-705.5
Timeless Science Period (gene) timeless RNA-binding protein Biology General Biochemistry Genetics and Molecular Biology alternative splicing 03 medical and health sciences 0302 clinical medicine RNA interference Circadian rhythm Biology (General) 030304 developmental biology 0303 health sciences D. melanogaster General Immunology and Microbiology General Neuroscience Alternative splicing RNA General Medicine Cell biology circadian rhythms RNA splicing Medicine RNA binding proteins 030217 neurology & neurosurgery Research Article Neuroscience |
| Zdroj: | eLife, Vol 8 (2019) eLife |
| ISSN: | 2050-084X |
| DOI: | 10.7554/elife.50063 |
| Popis: | The Drosophila circadian pacemaker consists of transcriptional feedback loops subjected to post-transcriptional and post-translational regulation. While post-translational regulatory mechanisms have been studied in detail, much less is known about circadian post-transcriptional control. Thus, we targeted 364 RNA binding and RNA associated proteins with RNA interference. Among the 43 hits we identified was the alternative splicing regulator P-element somatic inhibitor (PSI). PSI regulates the thermosensitive alternative splicing of timeless (tim), promoting splicing events favored at warm temperature over those increased at cold temperature. Psi downregulation shortens the period of circadian rhythms and advances the phase of circadian behavior under temperature cycle. Interestingly, both phenotypes were suppressed in flies that could produce TIM proteins only from a transgene that cannot form the thermosensitive splicing isoforms. Therefore, we conclude that PSI regulates the period of Drosophila circadian rhythms and circadian behavior phase during temperature cycling through its modulation of the tim splicing pattern. |
| Databáze: | OpenAIRE |
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