Production of Pleurotus sajor-caju crude enzyme broth and its applicability for the removal of bisphenol A
| Autor: | Elisabeth Wisbeck, Regina Maria Miranda Gern, Agenor Furigo Junior, Marcia Luciane Lange Silveira, Sandra Aparecida Furlan, Jamile Rosa Rampinelli, Theodoro Marcel Wagner, Auriciane Arbigaus, Mariane Bonatti-Chaves, Mahara Pereira de Melo |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Green chemistry
Basidiomycetes Bisphenol A Science 010501 environmental sciences Lentinula Pleurotus 01 natural sciences enzymatic activity Submerged culture Ferulic acid 03 medical and health sciences chemistry.chemical_compound Phenols Bioreactor Food science Benzhydryl Compounds 030304 developmental biology 0105 earth and related environmental sciences Laccase endocrine disruptor 0303 health sciences Multidisciplinary biology Chemistry Vanillin Banana wastes biology.organism_classification equipment and supplies |
| Zdroj: | Anais da Academia Brasileira de Ciências, Volume: 93, Issue: 1, Article number: e20191153, Published: 26 MAR 2021 Anais da Academia Brasileira de Ciências, Vol 93, Iss 1 (2021) Anais da Academia Brasileira de Ciências v.93 n.1 2021 Anais da Academia Brasileira de Ciências Academia Brasileira de Ciências (ABC) instacron:ABC |
| Popis: | Bisphenol A is an endocrine interfering compound, produced and used on a large scale worldwide. Chemical and biologic methods can be used to remove it from the environment. Biological methods are considered less costly, safer and, according to green chemistry definitions, an environmentally correct method. Considering the use of a crude enzyme broth, without any downstream process, the costs could be mostly reduced. Thus, the removal of bisphenol A by Pleurotus sajor-caju crude enzyme broth was investigated. Initially, the agro-industrial wastes were characterized and, the composition of the culture medium and the bioreactor culture conditions were defined. The enzyme produced in the highest concentration was characterized and the crude broth used in the bisphenol A removal assays. The OXI45 culture medium presented the highest laccase activity (1,850.7 U L-1, 350 rpm). Greater laccase stability was observed at 20 - 40 oC and pHs 5 - 7. Vanillin and ferulic acid (considered mediator compounds) were identified in the crude broth, probably helping on the obtention of the high value of removal effectiveness (0.052 mg U-1 h-1). The results indicate the potential use of the Pleurotus sajor-caju crude enzyme broth to obtain an enzymatic formulation for application in the environmental area. |
| Databáze: | OpenAIRE |
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