Phytochemical Analysis and Evaluation of Antioxidant and Biological Activities of Extracts from Three Clauseneae Plants in Northern Thailand
| Autor: | Pisit Poolprasert, Keerati Tanruean, Jaturong Kumla, Nakarin Suwannarach, Saisamorn Lumyong |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Murraya
DPPH Flavonoid antioxidant activity Plant Science Clausena excavata α-glucosidase inhibitory activity 01 natural sciences Article 03 medical and health sciences chemistry.chemical_compound DNA barcode volatile compounds Ecology Evolution Behavior and Systematics Anethole 030304 developmental biology chemistry.chemical_classification 0303 health sciences ABTS Ecology biology Traditional medicine Caryophyllene Botany ACE inhibitory activity biology.organism_classification 0104 chemical sciences 010404 medicinal & biomolecular chemistry anticancer activity Phytochemical chemistry QK1-989 tribe Clauseneae |
| Zdroj: | Plants Plants, Vol 10, Iss 117, p 117 (2021) Volume 10 Issue 1 |
| ISSN: | 2223-7747 |
| Popis: | This study established the DNA barcoding sequences (matK and rbcL) of three plant species identified in the tribe Clauseneae, namely Clausena excavata, C. harmandiana and Murraya koenigii. The total phenolic and total flavonoid contents, together with the biological activities of the derived essential oils and methanol extracts, were also investigated. Herein, the success of obtaining sequences of these plant using two different barcode genes matK and rbcL were 62.5% and 100%, respectively. Both regions were discriminated by around 700 base pairs and these had resemblance with those of the Clausenae materials earlier deposited in Genbank at a 99&ndash 100% degree of identity. Additionally, the use of matK DNA sequences could positively confirm the identity as monophyletic. The highest total phenolic and total flavonoid content values (p < 0.05) were observed in the methanol extract of M. koenigii at 43.50 mg GAE/g extract and 66.13 mg QE/g extract, respectively. Furthermore, anethole was detected as the dominant compound in C. excavata (86.72%) and C. harmandiana (46.09%). Moreover, anethole (26.02%) and caryophyllene (21.15%) were identified as the major phytochemical compounds of M. koenigii. In terms of the biological properties, the M. koenigii methanol extract was found to display the greatest amount of antioxidant activity (DPPH IC50 95.54 µ g/mL, ABTS value 118.12 mg GAE/g extract, FRAP value 48.15 mg GAE/g extract), and also revealed the highest &alpha glucosidase and antihypertensive inhibitory activities with percent inhibition values of 84.55 and 84.95. Notably, no adverse effects on human peripheral blood mononuclear cells were observed with regard to all of the plant extracts. Furthermore, M. koenigii methanol extract exhibited promise against human lung cancer cells almost at 80% after 24 h and 90% over 48 h. |
| Databáze: | OpenAIRE |
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