Association of 14-bp insertion/deletion polymorphism of HLA-G gene with idiopathic recurrent miscarriages in infertility center patients in Yazd, Iran
| Autor: | Fateme Arjmand, Morteza Samadi |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Adult
Abortion Habitual Immunology Human leukocyte antigen Biology Iran Toxicology law.invention 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Gene Frequency INDEL Mutation law Pregnancy HLA-G Recurrent miscarriage Genotype medicine Ultraviolet light Humans Allele frequency Polymerase chain reaction Alleles HLA-G Antigens 030219 obstetrics & reproductive medicine Polymorphism Genetic medicine.disease Molecular biology chemistry Case-Control Studies Female Ethidium bromide 030215 immunology |
| Zdroj: | Journal of immunotoxicology. 13(2) |
| ISSN: | 1547-6901 |
| Popis: | HLA-G is supposed to play a pivotal role in tolerance of the semi-allogeneic graft in pregnancy by inhibiting the cytotoxic functions of T and NK cells. A 14-bp insertion and/or deletion polymorphism in exon-8 has a possible role in HLA-G expression. The present study analyzed the 14-bp insertion/deletion polymorphism in normal pregnancy and recurrent miscarriage patients in order to discover a possible correlation between the 14-bp polymorphism and recurrent miscarriage (RM). In this study, genomic DNA from 200 RM patients and 200 normal fertile control individuals using the routine salting out method were isolated. Exon-8 of HLA-G gene of the two groups were amplified using polymerase chain reaction and analyzed by electrophoresis on 10% non-denaturing polyacrylamide gel electrophoresis containing ethidium bromide and visualized under ultraviolet light. HLA-G allele frequencies and genotypes in RM women and the fertile control group were compared using a Chi-square test. The results showed that there was a difference in allelic frequencies of 14-bp insertion polymorphism between fertile controls and RM patients; the frequency of +14 bp/-14 bp heterozygotes was significantly higher in RM patients as compared with fertile controls. Furthermore, the frequency of +14-bp insertion allele was significantly higher in those with RM as compared with normal fertile controls. From the findings here, it was concluded that a 14-bp insertion/deletion polymorphism in exon 8 could play a possible role in recurrent miscarriages. These results might ultimately be of significance for clinicians and those involved in understanding infertility and RM. |
| Databáze: | OpenAIRE |
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