Analysis of human tissues using Energy Dispersive X Ray Fluorescence – Dark matrix determination for the application to cancer research
| Autor: | Ana Rita Fialho Ensina, Diogo Casal, António Alberto Dias, Diogo Pais, Sofia Pessanha, José Paulo Santos, Jorge Machado, Maria Luisa de Carvalho, Patrícia Miguel da Silva Carvalho |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Analyte Matrix composition Calibration curve Chemistry X-Rays Ovary Analytical chemistry Spectrometry X-Ray Emission X-ray fluorescence Biochemistry Tumor tissue Trace Elements Inorganic Chemistry Matrix (chemical analysis) Certified reference materials Neoplasms Calibration Humans Molecular Medicine Female Energy (signal processing) |
| Zdroj: | Journal of Trace Elements in Medicine and Biology. 68:126837 |
| ISSN: | 0946-672X |
| DOI: | 10.1016/j.jtemb.2021.126837 |
| Popis: | Background X ray Fluorescence has been essayed as a suitable technique for the elemental quantification of trace element in human tissues, namely comparison of normal and cancerous tissue. However, accurate results depend on a robust quantification approach, namely correct evaluation of the samples’ dark matrix. Methods In order to determine the most suitable dark matrix composition for the quantification of such samples using the Fundamental Parameter approach, we have measured several Certified Reference Materials and essayed different dark matrix compositions to achieve the most accurate results. The resulting dark matrix was then applied to normal and tumor ovarian and prostate tissue samples, and the obtained results were compared with the ones obtained with a comparative method using external standard calibration curves. Results Using a dark matrix composed of 10 % - H, 22 % - C, 3 % - N and 60 % - O yielded the best compromise in accuracy for the light and heavy elements. For the reduced sample size and conditions of this study, for both organs, the concentrations of transition metals decrease in tumor tissues, while the concentration of lighter elements, P and Cl, increases. On the other hand, there are elements that showed different behavior between the two types of tissue, namely Zn and S, that increase in prostate tumor tissue and decrease in ovarian tissue. Conclusion An increase in precision was one of the improvements found with the newly developed method, as the FP-approach contemplates matrix effects and the influence of other elements in the analytes’ quantification. Additionally, the determined dark matrix can be employed in any tissue analysis application by means of EDXRF. |
| Databáze: | OpenAIRE |
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