Hormonal regulation and function of an RNA helicase, Ddx5 in corpus luteum of adult Wistar rats
| Autor: | Aparamita Pandey, Swati Rao, Killivalavan Asaithambi, Rudraiah Medhamurthy |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Prostaglandin Biology Chorionic Gonadotropin DEAD-box RNA Helicases Gonadotropin-Releasing Hormone 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Hormone Antagonists Corpus Luteum Pregnancy Internal medicine Luteolysis medicine Animals Rats Wistar 030219 obstetrics & reproductive medicine DDX5 Cloprostenol RNA Helicase A Rats Luteolytic Agents 030104 developmental biology medicine.anatomical_structure chemistry Gene Expression Regulation Cell culture Animal Science and Zoology Female Luteinizing hormone Corpus luteum Developmental Biology Hormone |
| Zdroj: | Reproductive biology. 19(2) |
| ISSN: | 2300-732X |
| Popis: | Corpus luteum (CL) is an endocrine tissue involved in regulation of reproductive cycle and early pregnancy establishment. In the present study DEAD-box helicase-5 (Ddx5), a member of the DEAD box family of RNA helicases was investigated for its expression, regulation and function in CL of Wistar rats. Ddx5 was expressed in adult rat CL. Primary cell culture from supra-ovulated ovaries were established for in vitro studies. Addition of luteinizing hormone (LH; 100 ng/ml), a luteotrophic factor in primary cell culture, decreased Ddx5 RNA expression (foldchange:0.6 ± 0.075) while prostaglandin alpha (PGF2α; 1μM), a luteolytic factor caused an increase (foldchange:2.4 ± 0.4) compared to control group. Under in vivo conditions, the administration of PGF2α or gonadotropin-releasing hormone antagonist; cetrorelix (CET) caused luteolysis as well as an increase in the protein level of Ddx5 (foldchange:1.9 ± 0.27 and 1.4 ± 0.09 viz.; p |
| Databáze: | OpenAIRE |
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