The forkhead transcription factor FOXK2 premarks lineage-specific genes in human embryonic stem cells for activation during differentiation
| Autor: | Andrew D. Sharrocks, Yaoyong Li, Sean X Liu, Zongling Ji |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Transcriptional Activation
Cell type Endoderm/cytology AcademicSubjects/SCI00010 Cell Differentiation/genetics Chromatin/metabolism 03 medical and health sciences 0302 clinical medicine Cell Lineage/genetics Genetics Forkhead Transcription Factors/metabolism Humans Transcription Factors/metabolism Enhancer Transcription factor Cells Cultured 030304 developmental biology Regulation of gene expression 0303 health sciences Embryonic Stem Cells/cytology biology Gene regulation Chromatin and Epigenetics Embryonic stem cell Chromatin Cell biology Histone Code Histone Enhancer Elements Genetic Regulatory sequence Mesoderm/cytology 030220 oncology & carcinogenesis biology.protein Neural Stem Cells/metabolism Neurogenesis/genetics |
| Zdroj: | Nucleic Acids Research Ji, Z, Li, Y, Liu, S X & Sharrocks, A 2021, ' The forkhead transcription factor FOXK2 premarks lineage-specific genes in human embryonic stem cells for activation during differentiation ', Nucleic acids research, vol. 49, no. 3, gkaa1281, pp. 1345-1363 . https://doi.org/10.1093/nar/gkaa1281 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gkaa1281 |
| Popis: | Enhancers play important roles in controlling gene expression in a choreographed spatial and temporal manner during development. However, it is unclear how these regulatory regions are established during differentiation. Here we investigated the genome-wide binding profile of the forkhead transcription factor FOXK2 in human embryonic stem cells (ESCs) and downstream cell types. This transcription factor is bound to thousands of regulatory regions in human ESCs, and binding at many sites is maintained as cells differentiate to mesendodermal and neural precursor cell (NPC) types, alongside the emergence of new binding regions. FOXK2 binding is generally associated with active histone marks in any given cell type. Furthermore newly acquired, or retained FOXK2 binding regions show elevated levels of activating histone marks following differentiation to NPCs. In keeping with this association with activating marks, we demonstrate a role for FOXK transcription factors in gene activation during NPC differentiation. FOXK2 occupancy in ESCs is therefore an early mark for delineating the regulatory regions, which become activated in later lineages. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|