Cardioprotective Solutions Exposure For 1 Hour in Hypoxia and Low Temperatures Affects Vascular Reactivity Differently
| Autor: | Maylla Ronacher Simões, Priscila Rossi de Batista, Dalton Valentim Vassallo, Melchior Luiz Lima |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Endothelium RD1-811 Heart preservation 030204 cardiovascular system & hematology Positive correlation Endothelium. Temperature 03 medical and health sciences Vascular reactivity Phenylephrine 0302 clinical medicine Internal medicine medicine Animals Diseases of the circulatory (Cardiovascular) system Vasoconstrictor Agents Rats Wistar Hypoxia Phenylephrine. Temperature business.industry Temperature General Medicine Hypoxia (medical) Rats Endocrinology medicine.anatomical_structure Vasoconstriction RC666-701 Original Article Surgery Endothelium Vascular medicine.symptom Cardiology and Cardiovascular Medicine business After treatment medicine.drug |
| Zdroj: | Brazilian Journal of Cardiovascular Surgery, Volume: 36, Issue: 2, Pages: 201-211, Published: 10 MAY 2021 Brazilian Journal of Cardiovascular Surgery, Vol 36, Iss 2, Pp 201-211 (2021) Brazilian Journal of Cardiovascular Surgery v.36 n.2 2021 Brazilian Journal of Cardiovascular Surgery Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV) instacron:SBCCV Brazilian Journal of Cardiovascular Surgery, Issue: ahead, Published: 28 APR 2021 |
| Popis: | Introduction: Heart preservation benefits cardiac performance after operations decreasing morbidity but the contribution of the vascular reactivity has been neglected. Objective: We evaluated whether cardioprotective solutions, Krebs-Henseleit (KH), Bretschneider-HTK (BHTK), St. Thomas No. 1 (STH-1), and Celsior (CEL), affect vascular reactivity. Methods: Aortic rings from Wistar rats were used in two protocols. First, the rings were exposed to BHTK, STH-1 or CEL for 1 hour of hypoxia at 37 °C. Second, the rings were exposed to 10 °C or 20 °C for 1 hour under hypoxia. After treatment, the rings were immersed in KH at 37 °C, endothelial integrity was tested and concentration-response curves to phenylephrine were performed. Results: In the first protocol, the solutions did not damage the endothelium; CEL and BHTK reduced KCl-induced contractions but not STH-1; only CEL and BHTK reduced vascular reactivity; there was a positive correlation between Rmax and KCl concentration. At 20 °C, 1 hour under hypoxia, the solutions produced similar KCl-induced contractions without endothelial damage. CEL, BHTK and STH-1 decreased vascular reactivity. At 10 °C, STH-1 increased reactivity but CEL and BHTK decreased. After 1 hour under hypoxia in CEL or BHTK solutions, reactivity was similar at different temperatures. At 20 °C, endothelial damage after exposure to STH-1 produced more vasoconstriction than CEL and BHTK. However, at 10 °C, endothelial damage after CEL and BHTK exposure elicited more vasoconstriction while STH-1 showed a small vasoconstrictor response, suggesting endothelial damage. Conclusion: STH-1 decreased reactivity at 20 °C and increased at 10 °C. CEL promoted greater endothelial modulation at 10 °C than at 20 °C, while STH-1 promoted higher modulation at 20 °C than at 10 °C. Vascular tone was reduced by CEL and BHTK exposure, also depending on the KCl concentration. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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