Detection of cytotoxic necrotising factor (CNF) in extracts of Escherichia coli strains by enzyme-linked immunosorbent assay
| Autor: | M. Tabouret, J. De Rycke |
|---|---|
| Přispěvatelé: | ProdInra, Migration, Station de pathologie de la reproduction [Nouzilly] (Unité 84), Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 1990 |
| Předmět: |
Microbiology (medical)
040301 veterinary sciences [SDV]Life Sciences [q-bio] Bacterial Toxins Enzyme-Linked Immunosorbent Assay Enterotoxin Cross Reactions medicine.disease_cause Microbiology 0403 veterinary science HeLa 03 medical and health sciences Sonication Predictive Value of Tests Freezing medicine Escherichia coli Animals Humans Cytotoxicity False Negative Reactions ComputingMilieux_MISCELLANEOUS 0303 health sciences biology 030306 microbiology Toxin Cytotoxins Escherichia coli Proteins Hemolysin 04 agricultural and veterinary sciences General Medicine biology.organism_classification Molecular biology Enterobacteriaceae [SDV] Life Sciences [q-bio] FACTEUR CYTOTOXIQUE NECROSANT Bacteria HeLa Cells |
| Zdroj: | Journal of Medical Microbiology Journal of Medical Microbiology, Society for General Microbiology, 1990, 32 (2), pp.73-81 |
| ISSN: | 0022-2615 1473-5644 |
| Popis: | An enzyme-linked immunosorbent assay (ELISA) consisting of a double sandwich technique with rabbit and sheep antibodies, was developed for the detection of cytotoxic necrotising factor (CNF) in extracts of Escherichia coli strains. The assay was evaluated by comparison with the results obtained with an assay based on toxicity for HeLa cell cultures. In a study of extracts of 27 CNF+ and 45 CNF- strains obtained by ultrasonic disintegration, no false positive and only three false negative results were recorded; the latter were obtained with strains that produced less CNF than any of the others examined. Frozen-thawed extracts contained about four times more CNF cytotoxic activity than extracts prepared ultrasonically; the testing of 54 CNF+ and 68 CNF- frozen-thawed extracts resulted in no false positive and only one false negative response. Whichever type of extract was used, no significant cross-reaction was observed with heat-labile (LT) or heat stable (ST) enterotoxin, verotoxin (VT1, VT2), haemolysin, or Vir cytotoxin. |
| Databáze: | OpenAIRE |
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