Extended and sequential delivery of protein from injectable thermoresponsive hydrogels
| Autor: | Zuwei Ma, Cory E. Leeson, Devin M. Nelson, William R. Wagner |
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| Rok vydání: | 2011 |
| Předmět: |
Materials science
Polymers Polyesters Biomedical Engineering Biocompatible Materials Methacrylate Article Injections Biomaterials chemistry.chemical_compound Drug Delivery Systems Tissue engineering Materials Testing Animals Bovine serum albumin Acrylic acid Acrylamides Drug Carriers biology Molecular Structure Metals and Alloys technology industry and agriculture Temperature Hydrogels Serum Albumin Bovine Microspheres Polyester Chemical engineering chemistry Acrylates Drug delivery Self-healing hydrogels Ceramics and Composites biology.protein Methacrylates Cattle Drug carrier Biomedical engineering |
| Zdroj: | Journal of biomedical materials research. Part A. 100(3) |
| ISSN: | 1552-4965 |
| Popis: | Thermoresponsive hydrogels are attractive for their injectability and retention in tissue sites where they may serve as a mechanical support and as a scaffold to guide tissue remodeling. Our objective in this report was to develop a thermoresponsive, biodegradable hydrogel system that would be capable of protein release from two distinct reservoirs – one where protein was attached to the hydrogel backbone, and one where protein was loaded into biodegradable microparticles mixed into the network. Thermoresponsive hydrogels consisting of N-isopropylacrylamide (NIPAAm), 2-hydroxyethyl methacrylate (HEMA), and biodegradable methacrylate polylactide (MAPLA) were synthesized along with modified copolymers incorporating 1 mol% protein-reactive methacryloxy N,hydroxysuccinimide (MANHS), hydrophilic acrylic acid (AAc), or both. In vitro, bovine serum albumin (BSA) release was studied from hydrogels, poly(lactide-co-glycolide) microparticles, or microparticles mixed into the hydrogels. The synthesized copolymers were able to gel below 37°C and release protein in excess of 3 months. The presence of MANHS and AAc in the copolymers was associated with higher loaded protein retention during thermal transition (45% vs 22%) and faster release (2 months), respectively. Microspheres entrapped in the hydrogel released protein in a delayed fashion relative to microspheres in saline. The combination of a protein-reactive hydrogel mixed with protein-loaded microspheres demonstrated a sequential release of specific BSA populations. Overall, the described drug delivery system combines the advantages of injectability, degradability, extended release, and sequential release which may be useful in tissue engineering applications. |
| Databáze: | OpenAIRE |
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