A simple method for absorption spectrophotometry of biological materials at low temperature
| Autor: | G.F. Doebbler, W.B. Elliott |
|---|---|
| Rok vydání: | 1965 |
| Předmět: |
Aqueous solution
Absorption spectroscopy medicine.diagnostic_test Nitrogen Chemistry Research Ice Temperature Analytical chemistry Liquid nitrogen Biochemistry Genetics and Molecular Biology (miscellaneous) Cold Temperature Cuvette chemistry.chemical_compound Boiling point Equipment and Supplies Spectrophotometry Freezing medicine Cytochromes Benzene Absorption (electromagnetic radiation) |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis. 94:317-323 |
| ISSN: | 0926-6585 |
| DOI: | 10.1016/0926-6585(65)90040-3 |
| Popis: | A simple procedure is described for using commercially available recording spectrophotometers for determining absorption spectra of aqueous samples at low temperature. In contrast to previous methods, very rapid initial freezing is employed followed by scanning of the specimen in a windowless metal cuvette maintained near the boiling point of liquid N2 in an inexpensive, easily constructed, polyurethane foam chamber. Sharpening, splitting, and intensification of absorption bands of cytochrome c and benzene in water are described. The windowless cuvette has been used for the 220–800-mμ wavelength range. |
| Databáze: | OpenAIRE |
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