Chitosan nanoparticles having higher degree of acetylation induce resistance against pearl millet downy mildew through nitric oxide generation
| Autor: | Andrei Mocan, Niranjan Raj Satyanarayana, Venkataramana Mudili, Vijai Kumar Gupta, Naveen Kumar Kalagatur, Rakesh K. Srivastava, Xiao Feng Dai, Bhim Singh, Keelara Veerappa Harish Prasanth, Chandra Nayaka Siddaiah, Jie Yin Chen, Tara Satyavati |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Pennisetum Science Sclerospora graminicola Germination Phenylalanine ammonia-lyase Nitric Oxide 01 natural sciences Benzoates Article Microbiology Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Disease Resistance Peroxidase Phenylalanine Ammonia-Lyase Plant Diseases Plant Proteins Chitosan Peronospora Multidisciplinary biology Superoxide Dismutase Imidazoles food and beverages Acetylation biology.organism_classification Catalase Plant disease 030104 developmental biology chemistry Seedlings Seed treatment Seeds biology.protein Downy mildew Nanoparticles Medicine Catechol Oxidase 010606 plant biology & botany |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-14 (2018) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Downy mildew of pearl millet caused by the biotrophic oomycete Sclerospora graminicola is the most devastating disease which impairs pearl millet production causing huge yield and monetary losses. Chitosan nanoparticles (CNP) were synthesized from low molecular weight chitosan having higher degree of acetylation was evaluated for their efficacy against downy mildew disease of pearl millet caused by Sclerospora graminicola. Laboratory studies showed that CNP seed treatment significantly enhanced pearl millet seed germination percentage and seedling vigor compared to the control. Seed treatment with CNP induced systemic and durable resistance and showed significant downy mildew protection under greenhouse conditions in comparison to the untreated control. Seed treatment with CNP showed changes in gene expression profiles wherein expression of genes of phenylalanine ammonia lyase, peroxidase, polyphenoloxidase, catalase and superoxide dismutase were highly upregulated. CNP treatment resulted in earlier and higher expression of the pathogenesis related proteins PR1 and PR5. Downy mildew protective effect offered by CNP was found to be modulated by nitric oxide and treatment with CNP along with NO inhibitors cPTIO completely abolished the gene expression of defense enzymes and PR proteins. Further, comparative analysis of CNP with Chitosan revealed that the very small dosage of CNP performed at par with recommended dose of Chitosan for downy mildew management. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |