Evaluation of Cartilage and Bone Degradation in a Murine Collagen Antibody-induced Arthritis Model
| Autor: | Morten A. Karsdal, K.E. Rasmussen, Per Qvist, Nancy Doyle, S. Oestergaard, Susan Y. Smith, Luc Chouinard, Aurore Varela |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Lipopolysaccharides
Male Pathology medicine.medical_specialty Lipopolysaccharide Immunology Anti-Inflammatory Agents Arthritis Bone and Bones Dexamethasone Mice chemistry.chemical_compound Adjuvants Immunologic Animals Medicine Collagen Type II Mice Inbred BALB C biology business.industry Cartilage General Medicine medicine.disease Arthritis Experimental medicine.anatomical_structure chemistry Collagen antibody induced arthritis biology.protein Experimental pathology Antibody Tomography X-Ray Computed business Joint lesions medicine.drug |
| Zdroj: | Scandinavian Journal of Immunology. 67:304-312 |
| ISSN: | 1365-3083 0300-9475 |
| DOI: | 10.1111/j.1365-3083.2007.02060.x |
| Popis: | The purpose of this work was to validate collagen antibody-induced arthritis (CAIA) model in two mice strains (Balb/c and CD-1) using clinical, biochemical, microstructural and histological techniques. We induced arthritis in mice using a cocktail of collagen type II (CII) antibodies followed by an injection with lipopolysaccharide (LPS) in different doses in Balb/c and CD-1 mice strains. Serum CTX-II levels were measured at study termination and correlated with microscopic severity of joint lesions as determined by a validated scoring systems. Bone involvement was assessed by microcomputer tomography (micro-CT). Balb/c mice developed rapid (day 6) and robust (100%) arthritis, whereas CD-1 mice showed only temporary macroscopic signs of disease. Serum CTX-II levels in Balb/c mice showed a significant increase in cartilage degradation in diseased animals (43-64% compared with non-diseased mice) and was decreased in animals receiving dexamethasone. Correlation of serum CTX-II with the microscopic score was statistically significant (P < 0.01). Micro-CT analysis demonstrated structural damage in bone in the CAIA Balb/c mice, which was prevented by dexamethasone. The CAIA-LPS model provides a useful supplement to currently available animal models of arthritis. This is a rapid onset and robust model; however, the choice of mouse strain should be evaluated carefully. |
| Databáze: | OpenAIRE |
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