Next-generation sequencing analysis reveals high bacterial diversity in wild venomous and non-venomous snakes from India

Autor: Jishnu Manazhi, Pradeep Kumar, Ravi Gupta, Sam Santhosh, Megha Muraleedharan, Bangaruswamy Dhinoth Kumar, Sajesh Puthenpurackal Krishnankutty, George Thomas, Jubina Benny, Rajadurai Chinnasamy Perumal, Arun Zachariah, Saju Michael, Bipin Balan
Rok vydání: 2018
Předmět:
Zdroj: Journal of Venomous Animals and Toxins including Tropical Diseases v.24 2018
The Journal of venomous animals and toxins including tropical diseases
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 24, Iss 1, Pp 1-11 (2018)
The Journal of Venomous Animals and Toxins Including Tropical Diseases
ISSN: 1678-9199
Popis: Background The oral cavities of snakes are replete with various types of bacterial flora. Culture-dependent studies suggest that some of the bacterial species are responsible for secondary bacterial infection associated with snakebite. A complete profile of the ophidian oral bacterial community has been unreported until now. Therefore, in the present study, we determined the complete bacterial compositions in the oral cavity of some snakes from India. Methods Total DNA was isolated from oral swabs collected from three wild snake species (Indian Cobra, King Cobra and Indian Python). Next, the DNA was subjected to PCR amplification of microbial 16S rRNA gene using V3-region-specific primers. The amplicons were used for preparation of DNA libraries that were sequenced on an Illumina MiSeq platform. Results The cluster-based taxonomy analysis revealed that Proteobacteria and Actinobacteria were the most predominant phyla present in the oral cavities of snakes. This result indicates that snakes show more similarities to birds than mammals as to their oral bacterial communities. Furthermore, our study reports all the unique and common bacterial species (total: 147) found among the oral microbes of snakes studied, while the majority of commonly abundant species were pathogens or opportunistic pathogens to humans. A wide difference in ophidian oral bacterial flora suggests variation by individual, species and geographical region. Conclusion The present study would provide a foundation for further research on snakes to recognize the potential drugs/antibiotics for the different infectious diseases. Electronic supplementary material The online version of this article (10.1186/s40409-018-0181-8) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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