Production of α-glucosidase inhibitor in the intestines by Bacillus licheniformis

Autor:	Deug-Chan Lee, Hae-Ik Rhee, Hee-Woong Kim
Rok vydání:	2022
Předmět:	biology Chemistry α glucosidase fungi Bioengineering biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Intestines Mice Animals Bacillus licheniformis Glycoside Hydrolase Inhibitors Food science Biotechnology
Zdroj:	Enzyme and Microbial Technology. 158:110032
ISSN:	0141-0229
Popis:	Alpha-glucosidase (EC.3.2.1.20) is involved in the absorption of monosaccharides in the small intestine of animals. We aimed to find a microorganism capable of proliferating in the intestine and producing α-glucosidase inhibitor. We developed a strain capable of forming spores from dry grass and growing in an anaerobic environment was selected as Bacillus lichenformis. Mixing spores of this strain with a high-fat diet and high-carbohydrate diet, it was confirmed that the weight gain was significantly reduced than the high-calorie diet group without spores. Furthermore it was confirmed that Bacillus lichenformis administered as spores efficiently proliferated in the intestine and consistently produced α-glucosidase inhibitor by securing a constant amount of the strain and α-glucosidase inhibitor in feces after a certain period. This study shows an efficient process in which microorganisms capable of proliferating in the intestine directly produce and supply specific secondary metabolites in the intestine.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6dc92a754f5a35de1d58585f7a35977 https://doi.org/10.1016/j.enzmictec.2022.110032 Zobrazit plný text záznamu Full Text from ScienceDirect