| Popis: |
Treatment of canine pulmonary membranes with detergent-free 8 M urea yielded a single class of large MW high-affinity binding sites exhibiting the stereospecificity and order of potency for beta-ligands characteristic of the beta receptor. This urea-solubilized receptor, demonstrating a 3–4 fold decreased affinity for beta-ligands as compared to membrane-bound and digitonin-solubilized receptor, exhibited a marked loss of activity when concomitantly exposed to solubilizing concentrations of detergent. |
