Seroprevalence of Equine Herpesvirus 1 (EHV-1) and Equine Herpesvirus 4 (EHV-4) in the Northern Moroccan Horse Populations
| Autor: | Farid Amraoui, Chafiqa Lotfi, Mohamed Dehhaoui, Mohammed Piro, Romain Paillot, Hanane El Ouadi, Barbara Colitti, Zineb El Brini, Ouafaa Fassi Fihri, Hassan Alyakine |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
General Veterinary
seroprevalence Veterinary medicine Equine herpesvirus 1 Antibody titer Horse Biology biology.organism_classification Virology EHV-4 Article Vaccination Titer Morocco EHV-1 QL1-991 SF600-1100 biology.protein VNT Seroprevalence Animal Science and Zoology ELISA Antibody Equine herpesvirus Zoology |
| Zdroj: | Animals : an Open Access Journal from MDPI Animals, Vol 11, Iss 2851, p 2851 (2021) Animals Volume 11 Issue 10 |
| ISSN: | 2076-2615 |
| Popis: | Simple Summary This work aims to evaluate the seroprevalence of equine EHV-1/4 in horse populations in the north of Morocco and to measure the antibody titers in vaccinated horses, under field conditions, with monovalent EHV-1 vaccines. Overall, 12.8% unvaccinated, and 21.8% vaccinated horses were positive for EHV-1. All samples were positive for EHV-4 when tested with the type-specific ELISA. The virus neutralization test showed low antibody titers in samples from vaccinated horses. Our study demonstrated that EHV-1 and EHV-4 are endemic in the horse populations in the north of Morocco and highlighted the necessity of reevaluating the vaccines and the vaccination protocol used. Abstract This study reports the first equine herpesvirus-1 (EHV-1) and equine herpesvirus-4 (EHV-4) seroprevalence investigation in horse populations of Morocco in 24 years. It also aims to determine antibody titers in horses vaccinated under field conditions with a monovalent EHV-1 vaccine. Blood samples were collected from 405 horses, including 163 unvaccinated and 242 vaccinated animals. They were tested using a commercial type-specific enzyme-linked immunosorbent assay (ELISA) and a virus neutralization test (VNT). Overall, 12.8% unvaccinated, and 21.8% vaccinated horses were positive for EHV-1. All samples were positive for EHV-4 when tested with the type-specific ELISA. In the vaccinated group, the VNT revealed a mean antibody titer of 1:49 for EHV-1 and 1:45 for EHV-4. The present study demonstrates that EHV-1 and EHV-4 are endemic in the horse populations in the north of Morocco, with prevalence differences between regions. Furthermore, horses vaccinated with a monovalent EHV-1 vaccine had low antibodies titers. This study highlights the necessity to establish and/or support efficient biosecurity strategies based on sound management of horses and characterize further and potentially improve the efficiency of the EHV vaccines and vaccination protocol used in the field. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|