Enhanced immune responses by skin vaccination with influenza subunit vaccine in young hosts
| Autor: | Sean R. McMaster, Timothy L. Denning, Dimitrios G. Koutsonanos, Ioanna Skountzou, Jacob E. Kohlmeier, E. Stein Esser, Jeong Woo Lee, Priya Kalluri, Richard W. Compans, Mark R. Prausnitz |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Injections
Intradermal Influenza vaccine T-Lymphocytes Population Antibodies Viral Injections Intramuscular Virus Article Immune system Drug Delivery Systems Influenza A Virus H1N1 Subtype Orthomyxoviridae Infections Immunology and Microbiology(all) Medicine Animals Antigen-presenting cell education Lung education.field_of_study Mice Inbred BALB C General Veterinary General Immunology and Microbiology business.industry Skin immunization Public Health Environmental and Occupational Health Germinal center Viral Load veterinary(all) Virology Survival Analysis Influenza 3. Good health Vaccination Infectious Diseases medicine.anatomical_structure Treatment Outcome Influenza Vaccines Immunoglobulin G Immunology Models Animal Vaccines Subunit Molecular Medicine Female Bone marrow business Vaccine |
| Zdroj: | Vaccine |
| ISSN: | 1873-2518 0264-410X |
| Popis: | Skin has gained substantial attention as a vaccine target organ due to its immunological properties, which include a high density of professional antigen presenting cells (APCs). Previous studies have demonstrated the effectiveness of this vaccination route not only in animal models but also in adults. Young children represent a population group that is at high risk from influenza infection. As a result, this group could benefit significantly from influenza vaccine delivery approaches through the skin and the improved immune response it can induce. In this study, we compared the immune responses in young BALB/c mice upon skin delivery of influenza vaccine with vaccination by the conventional intramuscular route. Young mice that received 5μg of H1N1 A/Ca/07/09 influenza subunit vaccine using MN demonstrated an improved serum antibody response (IgG1 and IgG2a) when compared to the young IM group, accompanied by higher numbers of influenza-specific antibody secreting cells (ASCs) in the bone marrow. In addition, we observed increased activation of follicular helper T cells and formation of germinal centers in the regional lymph nodes in the MN immunized group, rapid clearance of the virus from their lungs as well as complete survival, compared with partial protection observed in the IM-vaccinated group. Our results support the hypothesis that influenza vaccine delivery through the skin would be beneficial for protecting the high-risk young population from influenza infection. |
| Databáze: | OpenAIRE |
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