Endothelium-dependent vascular relaxation induced by Globularia alypum extract is mediated by EDHF in perfused rat mesenteric arterial bed
| Autor: | Aziz Chokri, Rachid Ben Cheikh, Kaouakib El Abida, Younes Filali Zegzouti |
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| Rok vydání: | 2012 |
| Předmět: |
Atropine
Male medicine.medical_specialty Endothelium Physiology Vasodilator Agents Indomethacin Vasodilation Endothelium dependent Nitric Oxide Globularia alypum Biological Factors Phenylephrine Bolus (medicine) Physiology (medical) Internal medicine Medicine Animals Enzyme Inhibitors Receptor Pharmacology business.industry Plant Extracts General Medicine Epoprostenol Receptors Muscarinic Mesenteric Arteries Rats medicine.anatomical_structure Endocrinology NG-Nitroarginine Methyl Ester Prostaglandin-Endoperoxide Synthases Potassium Female Endothelium Vascular Nitric Oxide Synthase business Scrophulariaceae medicine.drug |
| Zdroj: | Canadian journal of physiology and pharmacology. 90(5) |
| ISSN: | 1205-7541 |
| Popis: | The vasodilatory effect of Globularia alypum L. (GA) extract was evaluated in rat mesenteric arterial bed pre-contracted by continuous infusion of phenylephrine (2–4 ng/mL). Bolus injections of GA elicited dose–response vasodilation, which was abolished after endothelium removal. Addition of a nitric oxide synthase inhibitor, NG-nitro-l-arginine methyl ester (100 µmol/L), alone or in the presence of a cyclooxygenase inhibitor, indomethacin (10 µmol/L), did not significantly affect the vasodilation of the mesenteric arterial bed in response to GA extract. These results suggest that GA-induced vasodilation is endothelium dependent but nitric oxide and prostacyclin independent. In the presence of high K+ (60 mmol/L), the GA vasodilatory effect was completely abolished, suggesting that the vasodilation effect is mediated by hyperpolarization of the vascular cells. Also, pre-treatment with atropine (a muscarinic receptors antagonist) antagonized the GA-induced vasodilation, suggesting that the vasodilatory effect is mainly mediated by the endothelium-derived hyperpolarizing factor through activation of endothelial muscarinic receptors. |
| Databáze: | OpenAIRE |
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