Molecular Cloning and Characterization of a Novel Human Glycine-N-acyltransferase Gene GLYATL1, Which Activates Transcriptional Activity of HSE Pathway
| Autor: | Long Yu, Bo Wan, Guangming Ye, Zhaomin Zhong, Haoxing Zhang, Fang Xie, Qingyu Lang, Jie Li |
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| Rok vydání: | 2007 |
| Předmět: |
Glycine-N-acyltransferase
Biology Full Research Paper Catalysis lcsh:Chemistry Inorganic Chemistry GLYAT Exon GLYATL1 HSE pathway Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Gene Spectroscopy Organic Chemistry HEK 293 cells Intron General Medicine Subcellular localization Fusion protein Computer Science Applications Open reading frame lcsh:Biology (General) lcsh:QD1-999 Biochemistry |
| Zdroj: | International Journal of Molecular Sciences; Volume 8; Issue 5; Pages: 433-444 International Journal of Molecular Sciences, Vol 8, Iss 5, Pp 433-444 (2007) International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/i8050433 |
| Popis: | The glycine-N-acyltransferase (GLYAT) is well known to be involved in the detoxification of endogenous and exogenous xenobiotic acyl-CoA’s in mammals. Unfortunately, the knowledge about the gene encoding GLYAT is very limited. Here we report a novel gene encoding a GLYAT member, designated as GLYATL1, which was 1546 base pairs in length and contained an open reading frame (ORF) encoding a polypeptide of 302 amino acids. GLYATL1 was a split gene that was consisted of 7 exons and 6 introns and mapped to chromosome 11q12.1. The expression of GLYATL1 could be found in liver, kidney, pancreas, testis, ovary and stomach among 18 human tissues by RT-PCR analysis. Subcellular localization of myc-tagged GLYATL1 fusion protein revealed that GLYATL1 was distributed primarily in the cytoplasm of COS-7 cells. Furthermore, through the pathway profiling assay, the GLYATL1 protein was found to activate HSE signaling pathway in a dose-dependent manner when overexpressed in HEK293T cells. |
| Databáze: | OpenAIRE |
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