CD9 antigen is an accessory subunit of the VLA integrin complexes
| Autor: | Claude Boucheix, François Le Naour, Michel Prenant, Martine Billard, Eric Rubinstein |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Integrins
medicine.drug_class Immunology Integrin Molecular Sequence Data In Vitro Techniques Monoclonal antibody Transfection Tetraspanin 29 Mice L Cells Receptors Fibronectin Antigen Laminin Antigens CD Receptors Very Late Antigen Consensus Sequence medicine Immunology and Allergy Animals Amino Acid Sequence Immunologic Capping Antigen-presenting cell Cell Aggregation Membrane Glycoproteins biology Integrin beta1 hemic and immune systems Molecular biology Fibronectin Cell culture embryonic structures biology.protein Cell Adhesion Molecules |
| Zdroj: | European journal of immunology. 24(12) |
| ISSN: | 0014-2980 |
| Popis: | The CD9 antigen is a cell surface glycoprotein of unknown function which belongs to the tetraspans family. We demonstrate here, by precipitation, Western blotting and co-capping experiments, that this molecule is associated with a large fraction of beta 1 integrins in two cell lines, the pre-B cell line NALM-6 and the megakaryocytic cell line HEL. In HEL cells, CD9 antigen is only associated with VLA-4. In contrast, in NALM-6 cells, CD9 antigen is associated with both VLA-4 and VLA-5. On the other hand, only the beta 1 chain is co-precipitated with the CD9 antigen in transfected L cells. These data show that the CD9 antigen is associated with the beta 1 chain rather than with a particular integrin. CD9 monoclonal antibodies (mAb) did not modify the binding of HEL and NALM-6 cells to fibronectin, laminin or collagen. The association of CD9 antigen to VLA integrins is strengthened by the fact that both CD9 and anti-VLA mAb induce aggregation of the two cell lines and inhibit their migration in Transwell chambers. Because the aggregating effect, but not the inhibition of migration, is observed in CEM or CD9-transfected CEM cells, these two effects are likely to be mediated by different mechanisms. |
| Databáze: | OpenAIRE |
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