The effects of iloprost and alprostadil on ischemia-reperfusion injury in preventing inflammation, tissue degeneration, and apoptosis in rat skeletal muscle
| Autor: | İbrahim Özkan Alataş, Mustafa Behçet Sevin, Betül Can, Elif Güneysu, Cengiz Ovalı, Atacan Emre Kocman, Orhan Özatik |
|---|---|
| Přispěvatelé: | Kırşehir Ahi Evran Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri, Histoloji ve Embriyoloji ABD, Kırşehir Ahi Evran Üniversitesi |
| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
muscle Interleukin-1beta Ischemia Ischemia reperfusion injury iloprost alprostadil skeletal muscle Prostaglandin Inflammation Apoptosis Hindlimb Protective Agents Rats Sprague-Dawley chemistry.chemical_compound Health Care Sciences and Services Internal medicine Medicine Animals Iloprost Sağlık Bilimleri ve Hizmetleri Alprostadil Muscle Skeletal Cerrahi business.industry Tumor Necrosis Factor-alpha Skeletal muscle General Medicine medicine.disease Malondialdehyde skeletal Rats Endocrinology medicine.anatomical_structure chemistry Anesthesia Reperfusion Injury Female medicine.symptom business Oxidoreductases Reperfusion injury medicine.drug |
| Zdroj: | Volume: 47, Issue: 3 1028-1036 Turkish Journal of Medical Sciences |
| ISSN: | 1300-0144 1303-6165 |
| Popis: | Background/aim: The protective effects of prostaglandin (PG) analogs on ischemia-reperfusion (I/R) have been well documented; however, comparative studies are lacking. The aim of the present study was to determine whether iloprost or alprostadil is more effective in preventing muscle I/R injury. Materials and methods: Thirty-two rats were divided into four groups (n = 8): sham, control, IL (I/R + iloprost), and AL (I/R + alprostadil). I/R was induced by a tourniquet in the hindlimb for 3 h/3 h. The IL and AL groups received iloprost (0.5 ng kg–1 min–1) and alprostadil (0.05 µg kg–1 min–1) during reperfusion, respectively. After 6 h, blood and muscles were collected for analyses. Results: Serum TNF-α and IL-1? levels were decreased in the IL and AL groups compared with the control group (P < 0.05), whereas IL-6 levels did not change significantly. Tissue malondialdehyde levels were significantly lower in the IL and AL groups (P < 0.05). Tissue catalase levels showed no difference. The histological damage scores and apoptosis scores were both significantly decreased in the IL and AL groups compared with the control group (P< 0.05). Conclusion: The present study indicated that iloprost and alprostadil attenuated I/R injury in skeletal muscle. However, no comparable difference was evident regarding the efficacies of either PG analog. Background/aim: The protective effects of prostaglandin (PG) analogs on ischemia-reperfusion (I/R) have been well documented; however, comparative studies are lacking. The aim of the present study was to determine whether iloprost or alprostadil is more effective in preventing muscle I/R injury. Materials and methods: Thirty-two rats were divided into four groups (n = 8): sham, control, IL (I/R + iloprost), and AL (I/R + alprostadil). I/R was induced by a tourniquet in the hindlimb for 3 h/3 h. The IL and AL groups received iloprost (0.5 ng kg–1 min–1) and alprostadil (0.05 µg kg–1 min–1) during reperfusion, respectively. After 6 h, blood and muscles were collected for analyses. Results: Serum TNF-α and IL-1? levels were decreased in the IL and AL groups compared with the control group (P < 0.05), whereas IL-6 levels did not change significantly. Tissue malondialdehyde levels were significantly lower in the IL and AL groups (P < 0.05). Tissue catalase levels showed no difference. The histological damage scores and apoptosis scores were both significantly decreased in the IL and AL groups compared with the control group (P< 0.05). Conclusion: The present study indicated that iloprost and alprostadil attenuated I/R injury in skeletal muscle. However, no comparable difference was evident regarding the efficacies of either PG analog. |
| Databáze: | OpenAIRE |
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