Exploration and exploitation of the environment for novel specialized metabolites
| Autor: | Catarina Sales E Santos Loureiro, John van der Oost, Detmer Sipkema, Marnix H. Medema |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Bioinformatics metagenomic mining 030106 microbiology Biomedical Engineering Bioengineering Computational biology Environment Microbiology 03 medical and health sciences Data sequences Microbiologie SponGES Polyketide synthase Bioinformatica Life Science Peptide Synthases Gene VLAG 2. Zero hunger Horizon 2020 Bacteria biosynthetic gene cluster biology Deep-sea Sponge Ground Ecosystems of the North Atlantic: an integrated approach towards their preservation and sustainable exploitation Fungi heterologous expression Grant Agreement No 679849 Biosynthetic Pathways 030104 developmental biology Metagenomics Metabolome biology.protein EPS European Union (EU) Homologous recombination Nagoya Protocol Large size Biotechnology |
| Zdroj: | Current Opinion in Biotechnology, 50, 206-213 Current Opinion in Biotechnology Current Opinion in Biotechnology 50 (2018) |
| ISSN: | 0958-1669 |
| DOI: | 10.1016/j.copbio.2018.01.017 |
| Popis: | Microorganisms are Nature's little engineers of a remarkable array of bioactive small molecules that represent most of our new drugs. The wealth of genomic and metagenomic sequence data generated in the last decade has shown that the majority of novel biosynthetic gene clusters (BGCs) is identified from cultivation-independent studies, which has led to a strong expansion of the number of microbial taxa known to harbour BGCs. The large size and repeat sequences of BGCs remain a bioinformatic challenge, but newly developed software tools have been created to overcome these issues and are paramount to identify and select the most promising BGCs for further research and exploitation. Although heterologous expression of BGCs has been the greatest challenge until now, a growing number of polyketide synthase (PKS) and non-ribosomal peptide synthetase (NRPS)-encoding gene clusters have been cloned and expressed in bacteria and fungi based on techniques that mostly rely on homologous recombination. Finally, combining ecological insights with state-of-the-art computation and molecular methodologies will allow for further comprehension and exploitation of microbial specialized metabolites. Highlights • Environments that are most promising sources of new bioactives were identified. • New computational sequence binning tools improve large gene cluster reconstruction. • New cloning tools have led to the first successful heterologous expression of BGCs. |
| Databáze: | OpenAIRE |
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