Detection of Mycoplasma hyopneumoniae by ELISA and nested PCR from blood samples and nasal swabs from pigs in Slovakia
| Autor: | František Treml, Ľuboš Korytár, J. Süli, Anna Ondrejková, E. Slepecká, E. Haladová, D. Zendulková, Dávid Maženský, Zdenek Beníšek, M. Prokeš, Kateřina Rosenbergová, R. Ondrejka |
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| Rok vydání: | 2012 |
| Předmět: |
lcsh:Veterinary medicine
General Veterinary biology respiratory diseases Enzootic pneumonia animal diseases Respiratory disease medicine.disease biology.organism_classification Virology Mycoplasma hyopneumoniae Nasal Swab Diagnosis Herd biology.protein medicine antibodies lcsh:SF600-1100 Enzootic Antibody Nested polymerase chain reaction Pneumonia (non-human) |
| Zdroj: | Acta Veterinaria Brno, Vol 81, Iss 4, Pp 327-331 (2012) |
| ISSN: | 1801-7576 0001-7213 |
| Popis: | The aim of our study was to map the situation of swine mycoplasmoses on four farms in the region of Eastern Slovakia. The primary agent of Enzootic pneumonia of swine isMycoplasma hyopneumoniae. After reviewing the health status of conventional herds and evaluation of clinical symptoms, paired samples of nasal swabs and venous blood samples were collected from 38 pigs with clinical signs of respiratory disease. Nasal swab samples were tested by nested PCR, while blood samples were used to detect antibodies againstM. hyopneumoniaeby blockingELISA. The presence ofM. hyopneumoniaewas confirmed by nested PCR in four pigs (10.5%) and by blocking ELISA in 16 pigs (42.1%) of all four farms. This work presents for the first time comparison of different methods to diagnoseM. hyopneumoniaeinfection on pig farms in Eastern Slovakia. |
| Databáze: | OpenAIRE |
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