Regulatory network of two circRNAs and an miRNA with their targeted genes under astilbin treatment in pulmonary fibrosis
| Autor: | Guohong Cao, Xiaodong Song, Changjun Lv, Xiangyong Liu, Guangping Lu, Xiaoli Zhang, Jinjin Zhang, Pan Xu, Wenbo Liu, Minge Li |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Flavonols Drug action Computational biology Biology Transcriptome Bleomycin Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine GLI2 microRNA astilbin Animals Gene Regulatory Networks RNA-Seq Gene pulmonary fibrosis RNA RNA sequencing Original Articles RNA Circular Cell Biology Fibroblasts Gene Ontology 030104 developmental biology chemistry 030220 oncology & carcinogenesis Molecular Medicine Original Article Collagen Astilbin circRNAs Reprogramming |
| Zdroj: | Journal of Cellular and Molecular Medicine |
| ISSN: | 1582-4934 1582-1838 |
| DOI: | 10.1111/jcmm.14550 |
| Popis: | Circular RNAs (circRNAs) are becoming new therapeutic drug targets. However, their profiles under astilbin treatment have not been reported yet. In this study, we analysed the global reprogramming of circRNA transcriptome and a regulatory network of circRNAs with their targeted genes under astilbin treatment in pulmonary fibrosis. A total of 145 circRNAs were differentially expressed in the astilbin‐treated group compared with the bleomycin‐treated group using RNA sequencing. In the bleomycin‐ and astilbin‐treated groups, 29 coexpressed circRNAs were found. The maximum number of circRNAs was distributed on chromosome two, and their length varieties were mainly within 1000 bp. Four differentially expressed circRNAs (circRNA‐662, 949, 394 and 986) were tested to validate the RNA sequencing data, and their targeted microRNAs and genes were analysed by qRT‐PCR, Western blot, Pearson correlation coefficient, a dual‐luciferase reporter system and anti‐AGO2 RNA immunoprecipitation. The results showed that circRNA‐662 and 949 can act as “miR‐29b sponges” targeting Gli2 and STAT3 to exert their functions. Our work suggests that the transcriptome complexity at the circRNA level under astilbin treatment. These circRNAs may be potential molecular targets for drug action. |
| Databáze: | OpenAIRE |
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