Updated pigment composition of Tisochrysis lutea and purification of fucoxanthin using centrifugal partition chromatography coupled to flash chromatography for the chemosensitization of melanoma cells
| Autor: | Antoine Bonnet, Elodie Nicolau, Ahlem Jebali, Laurent Picot, Judith Rumin, Raimundo Gonçalves de Oliveira-Júnior, Raphaël Grougnet, Pierre-Edouard Bodet |
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| Přispěvatelé: | LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs), Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2020 |
| Předmět: |
Pigments
Multidrug resistance 01 natural sciences Isochrysis galbana Haptophyte chemistry.chemical_compound Column chromatography [SDV.SP.MED]Life Sciences [q-bio]/Pharmaceutical sciences/Medication Tisochrysis lutea Fucoxanthin 14. Life underwater [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Melanoma Carotenoid ComputingMilieux_MISCELLANEOUS chemistry.chemical_classification Natural product Chromatography biology 010405 organic chemistry Centrifugal partition chromatography biology.organism_classification Carotenoids 0104 chemical sciences 010404 medicinal & biomolecular chemistry chemistry Echinenone Chlorophyll Agronomy and Crop Science |
| Zdroj: | Algal Research-Biomass, Biofuels and Bioproducts Algal Research-Biomass, Biofuels and Bioproducts, Elsevier, 2020, 51, pp.102035. ⟨10.1016/j.algal.2020.102035⟩ Algal Research-biomass Biofuels And Bioproducts (2211-9264) (Elsevier BV), 2020-10, Vol. 51, P. 102035 (13p.) |
| ISSN: | 2211-9264 |
| Popis: | Tisochrysis lutea (T. lutea, ex T. Isochrysis galbana or T-Iso) is a marine haptophyte that was first isolated from Tahiti seawater. Because of its high content in lipids, this tropical species is commonly used in aquaculture to feed fishes, crustaceans and molluscs larvae. It is also a rich source of fucoxanthin with a high potential for nutraceutical, cosmetic and pharmaceutical applications. The purpose of the present study was to detail the pigment composition of T. lutea and to develop an efficient process to recover highly purified fucoxanthin. Using ultra performant liquid chromatography coupled to diode arrays and high-resolution mass spectrometry detectors (UPLC-DAD-MS/MS), we demonstrated for the first time the presence of echinenone, 3-hydroxy-echinenone and chlorophyll c2-monogalactosyldiacylglycerol [18:4/14:0] in unstressed cultures of T. lutea. The chemotaxonomic relevance of this updated pigment composition was discussed in relation to the Haptophyta phylum. A two-step purification of fucoxanthin was then optimized using centrifugal partition chromatography coupled to flash chromatography. This process allowed the efficient isolation of fucoxanthin (purity > 99%), that was further assessed as a low-toxicity antineoplastic and chemosensitizing natural product in human chemoresistant melanoma cells. This carotenoid exerted an antiproliferative activity in A2058 melanoma cells and reversed in vitro their chemoresistance to dacarbazine, a DNA-alkylating agent clinically used for the treatment of metastatic melanoma. |
| Databáze: | OpenAIRE |
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