Apoptosis Activation in Human Lung Cancer Cell Lines by a Novel Synthetic Peptide Derived from Conus californicus Venom
| Autor: | Karla Cervantes-Luevano, Irasema Oroz-Parra, Liliana N. Sánchez-Campos, Alexei F. Licea-Navarro, Mario Navarro, Guy S. Salvesen, Carolina Álvarez-Delgado |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Lung Neoplasms Health Toxicology and Mutagenesis lcsh:Medicine Mollusk Venoms Conus californicus Antineoplastic Agents Toxicology Article 03 medical and health sciences apoptotic-related genes synthetic peptide Cell Line Tumor Conus medicine Animals Humans Viability assay RNA Messenger Cytotoxicity Receptor Caspase bcl-2-Associated X Protein Caspase 7 biology pathway Caspase 3 lcsh:R apoptosis Conus Snail NF-kappa B Cancer biology.organism_classification medicine.disease Gene Expression Regulation Neoplastic lung cancer 030104 developmental biology Proto-Oncogene Proteins c-bcl-2 Apoptosis Cyclooxygenase 2 caspase-3 and -7 Immunology biology.protein Cancer research Peptides |
| Zdroj: | Toxins; Volume 8; Issue 2; Pages: 38 Toxins Toxins, Vol 8, Iss 2, p 38 (2016) |
| ISSN: | 2072-6651 |
| DOI: | 10.3390/toxins8020038 |
| Popis: | Lung cancer is one of the most common types of cancer in men and women and a leading cause of death worldwide resulting in more than one million deaths per year. The venom of marine snails Conus contains up to 200 pharmacologically active compounds that target several receptors in the cell membrane. Due to their diversity and specific binding properties, Conus toxins hold great potential as source of new drugs against cancer. We analyzed the cytotoxic effect of a 17-amino acid synthetic peptide (s-cal14.1a) that is based on a native toxin (cal14.1a) isolated from the sea snail Conus californicus. Cytotoxicity studies in four lung cancer cell lines were complemented with measurement of gene expression of apoptosis-related proteins Bcl-2, BAX and the pro-survival proteins NFκB-1 and COX-2, as well as quantification of caspase activity. Our results showed that H1299 and H1437 cell lines treated with s-call4.1a had decreased cell viability, activated caspases, and reduced expression of the pro-survival protein NFκB-1. To our knowledge, this is the first report describing activation of apoptosis in human lung cancer cell lines by s-cal14.1a and we offer insight into the possible mechanism of action. |
| Databáze: | OpenAIRE |
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