Recombinant interleukin-5 induces in vivo airway hyperresponsiveness to histamine in guinea pigs
| Autor: | Gerard A. Hofman, Huub F. J. Savelkoul, Frans P. Nijkamp, Ingrid van Ark, Antoon J.M. Van Oosterhout |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Respiratory airways
medicine.medical_treatment Guinea Pigs Cell Count Recombinant Interleukin Pharmacology Transfection Cell Line Guinea pig chemistry.chemical_compound Airway resistance In vivo medicine Animals Eosinophilia Interleukin 5 Asthma biology medicine.diagnostic_test business.industry Airway Resistance Antibodies Monoclonal Haplorhini respiratory system medicine.disease Recombinant Proteins respiratory tract diseases Eosinophils Cytokine Bronchoalveolar lavage chemistry Immunology biology.protein Hyperresponsiveness Bronchial Hyperreactivity Interleukin-5 Antibody medicine.symptom business Bronchoalveolar Lavage Fluid Injections Intraperitoneal Histamine |
| Zdroj: | European Journal of Pharmacology, 236(3), 379-383 European Journal of Pharmacology 236 (1993) 3 |
| ISSN: | 0014-2999 |
| DOI: | 10.1016/0014-2999(93)90475-w |
| Popis: | Interleukin-5-producing CV-1 cells were encapsulated in alginate and injected i.p. in guinea pigs (4 × 10 6 /animal). These cells produced approximately 8 ng interleukin-5 per 4 × 10 6 cells per day. Airway hyperresponsiveness to histamine in vivo was observed 3 and 7 days after administration. The increase in lung resistance after intravenous administration of histamine to guinea pigs was significantly potentiated, by approximately 70 to 90% in interleukin-5-treated animals. In animals treated with antibody to interleukin-5, the administration of interleukin-5-producing CV-1 cells did not induce hyperresponsiveness. The percentage of eosinophils in broncho-alveolar lavage fluid was increased by 100% at 7 days but not at 3 days after administration of interleukin-5-producing CV-1 cells. Antibody to interleukin-5 prevented the broncho-alveolar lavage eosinophilia at 7 days after interleukin-5 administration. It can be concluded that interleukin-5- induces broncho-alveolar lavage eosinophilia and airway hyperresponsiveness and that these phenomena do not occur simultaneously. These data suggest a role for interleukin-5 in the development of airway hyperresponsiveness in bronchial asthma. |
| Databáze: | OpenAIRE |
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