Bacterial resistance following subgingival and systemic administration of minocycline
| Autor: | Jorgen Lassen, Anne Merete Aass, Hans R. Preus, Sebastian G. Ciancio |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
Male medicine.medical_specialty Gingival and periodontal pocket medicine.drug_class Administration Topical Antibiotics Colony Count Microbial Administration Oral Minocycline Microbial Sensitivity Tests Gastroenterology Microbiology Agar plate Antibiotic resistance Internal medicine medicine Humans Periodontal Pocket Periodontitis Analysis of Variance Bacteria biology Tetracycline Resistance Streptococcus Middle Aged biology.organism_classification Systemic administration Periodontics Female Anaerobic exercise medicine.drug |
| Zdroj: | Journal of Clinical Periodontology. 22:380-384 |
| ISSN: | 1600-051X 0303-6979 |
| DOI: | 10.1111/j.1600-051x.1995.tb00164.x |
| Popis: | The aim of the present study was to compare total numbers of cultivable bacteria and prevalence of resistance to minocycline among periodontal bacteria following subgingival or systemic application of minocycline in patients suffering from periodontal disease. 10 adult patients were administered 2% minocycline ointment subgingivally into their periodontal pockets at baseline, week 2 and months 1,3,6 and 9. Patients had scaling/root planing at baseline and month 6. In addition, 10 patients undergoing scaling/root planing followed by a 10-day course of systemic minocycline therapy, were studied and compared with the subgingival application group. Bacterial samples were taken from the 4 deepest pockets before each subgingival application of the drug. The systemic administration group was sampled at baseline and at week 2, as well as months 1 and 3 after completing the antibiotic treatment. For each patient at each sampling, bacterial samples were pooled, diluted, seeded on parallel blood agar plates and incubated aerobically and anaerobically. After incubation, 30 colonies were picked at random and transferred to blood agar plates supplemented with 10 μg/ml minocycline, to estimate prevalence of minocycline-resistant bacteria. The results of this study indicate that subgingival application of minocycline ointment resulted in an initial reduction in total numbers of cultivable bacteria, which then remained depressed during the full year of the study. No such observation was made in the systemic administration. Both in the subgingival and the systemic administration group, the % of cultivable aerobic and anaerobic minocycline-resistant bacterial strains increased transiently following administration of the drug, but returned to baseline levels within 3 months post-treatment. The median of cultivable aerobic minocycline-resistant bacterial strains decreased or remained unchanged in both groups during the study periods. Thus, subgingival application of minocycline seems no more likely than systemic administration of the drug to select for an increased proportion of resistant organisms in the periodontal pockets. |
| Databáze: | OpenAIRE |
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