Comparison between thaw-mounting and use of conductive tape for sample preparation in ToF-SIMS imaging of lipids in Drosophila microRNA-14 model
| Autor: | Jin Gyeong Son, Sung Bae Lee, Minh Uyen Thi Le, Jeong Hyang Park, Tae Geol Lee, Hyun Kyoung Shon |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Analyte Materials science General Physics and Astronomy Spectrometry Mass Secondary Ion 01 natural sciences General Biochemistry Genetics and Molecular Biology Biomaterials 03 medical and health sciences Image Processing Computer-Assisted Animals General Materials Science Sample preparation Electrical conductor Image resolution Histocytological Preparation Techniques 010401 analytical chemistry Molecular biophysics Reproducibility of Results General Chemistry Tissue shrinkage Mass spectrometric Lipids 0104 chemical sciences Secondary ion mass spectrometry MicroRNAs 030104 developmental biology Drosophila Gene Deletion Biomedical engineering |
| Zdroj: | Biointerphases. 13(3) |
| ISSN: | 1559-4106 |
| Popis: | Time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging elucidates molecular distributions in tissue sections, providing useful information about the metabolic pathways linked to diseases. However, delocalization of the analytes and inadequate tissue adherence during sample preparation are among some of the unfortunate phenomena associated with this technique due to their role in the reduction of the quality, reliability, and spatial resolution of the ToF-SIMS images. For these reasons, ToF-SIMS imaging requires a more rigorous sample preparation method in order to preserve the natural state of the tissues. The traditional thaw-mounting method is particularly vulnerable to altered distributions of the analytes due to thermal effects, as well as to tissue shrinkage. In the present study, the authors made comparisons of different tissue mounting methods, including the thaw-mounting method. The authors used conductive tape as the tissue-mounting material on the substrate because it does not require heat from the finger for the tissue section to adhere to the substrate and can reduce charge accumulation during data acquisition. With the conductive-tape sampling method, they were able to acquire reproducible tissue sections and high-quality images without redistribution of the molecules. Also, the authors were successful in preserving the natural states and chemical distributions of the different components of fat metabolites such as diacylglycerol and fatty acids by using the tape-supported sampling in microRNA-14 (miR-14) deleted Drosophila models. The method highlighted here shows an improvement in the accuracy of mass spectrometric imaging of tissue samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|