Combined TLR/CD40 Stimulation Mediates Potent Cellular Immunity by Regulating Dendritic Cell Expression of CD70 In Vivo
| Autor: | Phillip J. Sanchez, Jennifer A. McWilliams, Hideo Yagita, Catherine Haluszczak, Ross M. Kedl |
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| Rok vydání: | 2007 |
| Předmět: |
Agonist
Cellular immunity medicine.drug_class Immunology Stimulation CD8-Positive T-Lymphocytes Mice medicine Animals Immunology and Allergy Cytotoxic T cell CD40 Antigens Cell Proliferation Immunity Cellular CD40 biology Chemistry Toll-Like Receptors Dendritic Cells Dendritic cell Acquired immune system Cell biology Mice Inbred C57BL Gene Expression Regulation biology.protein Immunization CD8 CD27 Ligand |
| Zdroj: | The Journal of Immunology. 178:1564-1572 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | We previously showed that immunization with a combination of TLR and CD40 agonists (combined TLR/CD40 agonist immunization) resulted in an expansion of Ag-specific CD8 T cells exponentially greater than the expansion observed to immunization with either agonist alone. We now show that the mechanism behind this expansion of T cells is the regulated expression of CD70 on dendritic cells. In contrast to previous results in vitro, the expression of CD70 on dendritic cells in vivo requires combined TLR/CD40 stimulation and is not significantly induced by stimulation of either pathway alone. Moreover, the exponential expansion of CD8+ T cells following combined TLR/CD40 agonist immunization is CD70 dependent. Thus, the transition from innate stimuli (TLRs) to adaptive immunity is controlled by the regulated expression of CD70. |
| Databáze: | OpenAIRE |
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