Effects in broiler hens of genetic lines differing in fertility, biotin supplementation, and age on relative abundance of oviductal transforming growth factor-β and carbonic anhydrase mRNA transcripts
| Autor: | S. Rostami, M.R. Zahedi, P. Rafiee, Mahdi Zhandi, Y. Jafari Ahangari, Z. Ansari Pirsaraei, H. Daryabari, F. Saemi, A. Akhlaghi, M. Bagheri, E. D. Peebles, A.N. Eghbalian |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Aging
media_common.quotation_subject Biotin Oviducts Biology Breeding Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Food Animals Abundance (ecology) Transforming Growth Factor beta Carbonic anhydrase Animals RNA Messenger Relative species abundance media_common Carbonic Anhydrases Messenger RNA 030219 obstetrics & reproductive medicine Reproduction 0402 animal and dairy science Broiler Age Factors 04 agricultural and veterinary sciences General Medicine 040201 dairy & animal science Pedigree Fertility chemistry Gene Expression Regulation Dietary Supplements biology.protein Animal Science and Zoology Animal Nutritional Physiological Phenomena Female Chickens Transforming growth factor |
| Zdroj: | Animal reproduction science. 219 |
| ISSN: | 1873-2232 |
| Popis: | There was evaluation of effects of biotin administration on oviductal abundance of transforming growth factor-β (TGF-β) and carbonic anhydrase (CA) mRNA transcript in younger and older broiler hens of relatively lesser and greater fertility lines. Additionally, effects of biotin supplementation on attenuation of age-related subfertility were evaluated. Hens from the relatively greater (Line D, n = 60) and lesser (Line B, n = 60) fertility rate line were randomly assigned to three treatment groups. Biotin was not or was administered in drinking water from 30 to 33 (younger age) and 53 to 56 (older age) wk of age to have access to no biotin (T0), or 0.3 (T1), or 0.45 (T2) mg/L of biotin. There was assessment the relative oviductal abundances of TGF-β and CA mRNA transcript abundances. Supplemental biotin and age had no effect on the relative abundance of oviductal TGF-β mRNA transcript in hens of Line D. There, however, was a ten-fold greater abundance of TGF-β in hens of the T0 group of Line B compared with Line D. Relative abundance of TGF-β mRNA transcript was greater in younger hens of Line B; however, biotin supplementation of older hens of the T2 group of Line B resulted in a similar TGF-β abundance to that of younger hens. Inconstant with the TGF-β abundance, CA abundance in hens of Line B was not affected by supplemental biotin or bird age. Overall, differences in TGF-β or CA abundances did not affect fertility of broiler hens. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect