The SLC19A1 80GA polymorphism is not associated with male infertility
Autor: | Marek Drozdzik, Wanda Górnik, Joanna Stefankiewicz, Tomasz Baczkowski, Mateusz Kurzawski, Rafał Kurzawa |
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Rok vydání: | 2010 |
Předmět: |
Infertility
Adult Male medicine.medical_specialty Adolescent Genotype Health Toxicology and Mutagenesis Clinical Biochemistry Biochemistry Polymerase Chain Reaction Male infertility Reduced Folate Carrier Protein Internal medicine medicine Odds Ratio Humans Allele Infertility Male Polymorphism Genetic biology Haplotype Cell Membrane Case-control study Age Factors Membrane Transport Proteins Middle Aged medicine.disease Molecular biology Endocrinology Methylenetetrahydrofolate reductase Case-Control Studies biology.protein Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
Zdroj: | Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals. 15(3) |
ISSN: | 1366-5804 |
Popis: | Previous studies have revealed that genetic factors may be involved in regulating folate turnover, e.g. methylenetetrahydrofolate reductase polymorphism in the development of male infertility. Folate transporter, encoded by the SLC19A1 gene, commonly referred to as reduced folate carrier (RFC) is a transmembrane protein, which transfers hydrophilic folates across the cell membrane. It was hypothesized that common polymorphism within the SLC19A1 gene (rs1051266:G>A, 80G>A) may alter RFC function. The aim of this study was to investigate a potential association between the SLC19A1 80G>A polymorphism and male infertility in a case-control study. The SLC19A1 80G>A polymorphism was determined by means of a polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) assay in 213 infertile Caucasian men and 226 ethnically matched controls. The distribution of SLC19A1 genotypes in the infertile men was as follows: GG 26.8%, GA 51.2%, AA 22.1% and in fertile men: GG 24.8%, GA 50.4%, AA 24.8%, and was comparable in the both the evaluated groups. Odds ratios (95% confidence interval, CI): 0.90 (0.59-1.38) and 0.88 (0.56-1.36) for dominant and recessive models remained non-significant, also after adjustment for age: 0.89 (0.57-1.37) and 0.80 (0.51-1.25), respectively. Our study demonstrated that polymorphism 80G>A of the SLC19A1 gene is not associated with male infertility. |
Databáze: | OpenAIRE |
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