Preferential binding of a stable G3BP ribonucleoprotein complex to intron-retaining transcripts in mouse brain and modulation of their expression in the cerebellum

Autor: Jamal Tazi, Eduardo Eyras, Marion de Toledo, Karim Chebli, Nicolás Bellora, Juan González-Vallinas, Henning Urlaub, Manuel Irimia, Sophie Martin, Monika Raabe, Ben J. Blencowe
Přispěvatelé: Adaptation et diversité en milieu marin (AD2M), Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales [Bariloche] (IPATEC), Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] (CONICET)-Universidad Nacional del Comahue [Neuquén] (UNCOMA), Barcelona Institute of Science and Technology (BIST), Institut de Génétique Moléculaire de Montpellier (IGMM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Centre de recherche en Biologie cellulaire de Montpellier (CRBM), Institució Catalana de Recerca i Estudis Avançats (ICREA), Max Planck Institute for Biophysical Chemistry (MPI-BPC), Max-Planck-Gesellschaft, Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Centre de recherche en Biologie Cellulaire (CRBM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Transcription
Genetic
[SDV]Life Sciences [q-bio]
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
Biochemistry
G3BP
Mice
Cerebellum
Poly-ADP-Ribose Binding Proteins
3' Untranslated Regions
ComputingMilieux_MISCELLANEOUS
Ribonucleoprotein
Regulation of gene expression
Mice
Knockout
Mice
Inbred BALB C
High-Throughput Nucleotide Sequencing
RETAINED INTRON
Immunohistochemistry
DNA-Binding Proteins
Cross-Linking Reagents
RNA Recognition Motif Proteins
[SDV.IMM]Life Sciences [q-bio]/Immunology
RNA
Long Noncoding
Ubiquitin Thiolesterase
RNA Helicases
CIENCIAS NATURALES Y EXACTAS
Otras Ciencias Biológicas
[SDV.CAN]Life Sciences [q-bio]/Cancer
Biology
Cytoplasmic Granules
Ciencias Biológicas
03 medical and health sciences
Cellular and Molecular Neuroscience
Splicing factor
Stress granule
Animals
CEREBELLUM
Brain Chemistry
Three prime untranslated region
Intron
DNA Helicases
STRESS GRANULES
RNA
Molecular biology
Introns
HITS-CLIP
Alcohol Oxidoreductases
030104 developmental biology
Gene Expression Regulation
Carrier Proteins
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Zdroj: Journal of Neurochemistry
Journal of Neurochemistry, 2016, 139 (3), pp.349-368. ⟨10.1111/jnc.13768⟩
Journal of Neurochemistry, Wiley, 2016, 139 (3), pp.349-368. ⟨10.1111/jnc.13768⟩
ISSN: 0022-3042
1471-4159
Popis: Neuronal granules play an important role in the localization and transport of translationally silenced messenger ribonucleoproteins in neurons. Among the factors associated with these granules, the RNA-binding protein G3BP1 (stress-granules assembly factor) is involved in neuronal plasticity and is induced in Alzheimer's disease. We immunopurified a stable complex containing G3BP1 from mouse brain and performed high-throughput sequencing and cross-linking immunoprecipitation to identify the associated RNAs. The G3BP-complex contained the deubiquitinating protease USP10, CtBP1 and the RNA-binding proteins Caprin-1, G3BP2a and splicing factor proline and glutamine rich, or PSF. The G3BP-complex binds preferentially to transcripts that retain introns, and to non-coding sequences like 3’-untranslated region and long non-coding RNAs. Specific transcripts with retained introns appear to be enriched in the cerebellum compared to the rest of the brain and G3BP1 depletion decreased this intron retention in the cerebellum of G3BP1 knockout mice. Among the enriched transcripts, we found an overrepresentation of genes involved in synaptic transmission, especially glutamate-related neuronal transmission. Notably, G3BP1 seems to repress the expression of the mature Grm5 (metabotropic glutamate receptor 5) transcript, by promoting the retention of an intron in the immature transcript in the cerebellum. Our results suggest that G3BP is involved in a new functional mechanism to regulate non-coding RNAs including intron-retaining transcripts, and thus have broad implications for neuronal gene regulation, where intron retention is widespread. Fil: Martin, Sophie. Institut de Génétique Moléculaire de Montpellier; Fiyi. Centre National de la Recherche Scientifique; Francia Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales.; Argentina Fil: González Vallinas, Juan. Universitat Pompeu Fabra; España Fil: Irimia, Manuel. University of Toronto; Canadá Fil: Chebli, Karim. Institut de Génétique Moléculaire de Montpellier; Fiyi. Centre National de la Recherche Scientifique; Francia Fil: de Toledo, Marion. Institut de Génétique Moléculaire de Montpellier; Fiyi. Centre National de la Recherche Scientifique; Francia Fil: Raabe, Monika. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Eyras, Eduardo. Institució Catalana de Recerca i Estudis Avancats; España. Universitat Pompeu Fabra; España Fil: Urlaub, Henning. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Blencowe, Ben J.. University of Toronto; Canadá Fil: Tazi, Jamal. Institut de Génétique Moléculaire de Montpellier; Fiyi. Centre National de la Recherche Scientifique; Francia
Databáze: OpenAIRE
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