Inhibitory Effects of Human Primary Intervertebral Disc Cells on Human Primary Osteoblasts in a Co-Culture System
| Autor: | Christoph E. Albers, Daniela Angelika Frauchiger, Lorin Michael Benneker, Rahel Deborah May, Sandro Kohl, Benjamin Gantenbein |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine medicine.medical_treatment Gene Expression lcsh:Chemistry 0302 clinical medicine Gene expression Intervertebral Disc 610 Medicine & health lcsh:QH301-705.5 Cells Cultured Spectroscopy Aged 80 and over Chemistry General Medicine musculoskeletal system human primary osteoblasts Computer Science Applications Cell biology medicine.anatomical_structure Spinal fusion Alkaline phosphatase Female Adult musculoskeletal diseases Nucleus Pulposus BMP antagonists Inhibitory postsynaptic potential Bone morphogenetic protein Article Catalysis Inorganic Chemistry 03 medical and health sciences Calcification Physiologic Matrix Metalloproteinases Secreted medicine Humans Secretion Physical and Theoretical Chemistry human primary intervertebral disc cells co-culture spinal fusion Molecular Biology Aged Osteoblasts Organic Chemistry Annulus Fibrosus Intervertebral disc Alkaline Phosphatase medicine.disease Coculture Techniques Pseudarthrosis 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 570 Life sciences biology 030217 neurology & neurosurgery |
| Zdroj: | International Journal of Molecular Sciences, Vol 19, Iss 4, p 1195 (2018) International Journal of Molecular Sciences May, Rahel Deborah; Frauchiger, Daniela Angelika; Albers, Christoph; Benneker, Lorin Michael; Kohl, Sandro; Gantenbein, Benjamin (2018). Inhibitory Effects of Human Primary Intervertebral Disc Cells on Human Primary Osteoblasts in a Co-Culture System. International journal of molecular sciences, 19(4) Molecular Diversity Preservation International MDPI 10.3390/ijms19041195 International Journal of Molecular Sciences; Volume 19; Issue 4; Pages: 1195 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms19041195 |
| Popis: | Spinal fusion is a common surgical procedure to address a range of spinal pathologies, like damaged or degenerated discs. After the removal of the intervertebral disc (IVD), a structural spacer is positioned followed by internal fixation, and fusion of the degenerated segment by natural bone growth. Due to their osteoinductive properties, bone morphogenetic proteins (BMP) are applied to promote spinal fusion. Although spinal fusion is successful in most patients, the rates of non-unions after lumbar spine fusion range from 5% to 35%. Clinical observations and recent studies indicate, that the incomplete removal of disc tissue might lead to failure of spinal fusion. Yet, it is still unknown if a secretion of BMP antagonists in intervertebral disc (IVD) cells could be the reason of inhibition in bone formation. In this study, we co-cultured human primary osteoblasts (OB) and IVD cells i.e., nucleus pulposus (NPC), annulus fibrosus (AFC) and cartilaginous endplate cells (CEPC), to test the possible inhibitory effect from IVD cells on OB. Although we could see a trend in lower matrix mineralization in OB co-cultured with IVD cells, results of alkaline phosphatase (ALP) activity and gene expression of major bone genes were inconclusive. However, in NPC, AFC and CEPC beads, an up-regulation of several BMP antagonist genes could be detected. Despite being able to show several indicators for an inhibition of osteoinductive effects due to IVD cells, the reasons for pseudarthrosis after spinal fusion remain unclear. |
| Databáze: | OpenAIRE |
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