MicroRNA expression signature in human abdominal aortic aneurysms
| Autor: | Irene Hinterseher, James R. Elmore, John L. Gray, Matthew C Pahl, Charles M. Schworer, David P. Franklin, David J. Carey, Helena Kuivaniemi, Thomas C. Peeler, Gerard Tromp, Gabor Gäbel, Kimberly Derr |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
lcsh:Internal medicine Microarray lcsh:QH426-470 Apoptosis macromolecular substances 030204 cardiovascular system & hematology Biology Bioinformatics Real-Time Polymerase Chain Reaction Vascular biology 03 medical and health sciences 0302 clinical medicine Gene expression microRNA Genetics Humans Genetics(clinical) Gene Regulatory Networks RNA Messenger lcsh:RC31-1245 Genetics (clinical) 030304 developmental biology Aged Oligonucleotide Array Sequence Analysis Regulation of gene expression 0303 health sciences Microarray analysis techniques Gene Expression Profiling miRNA-mRNA analysis Reproducibility of Results Microarray analysis Middle Aged Gene expression profiling MicroRNAs lcsh:Genetics Real-time polymerase chain reaction Gene Expression Regulation cardiovascular system Female Network analysis DNA microarray Research Article Aortic Aneurysm Abdominal |
| Zdroj: | BMC Medical Genomics, Vol 5, Iss 1, p 25 (2012) BMC Medical Genomics |
| ISSN: | 1755-8794 |
| Popis: | Background Abdominal aortic aneurysm (AAA) is a dilatation of the aorta affecting most frequently elderly men. Histologically AAAs are characterized by inflammation, vascular smooth muscle cell apoptosis, and extracellular matrix degradation. The mechanisms of AAA formation, progression, and rupture are currently poorly understood. A previous mRNA expression study revealed a large number of differentially expressed genes between AAA and non-aneurysmal control aortas. MicroRNAs (miRNAs), small non-coding RNAs that are post-transcriptional regulators of gene expression, could provide a mechanism for the differential expression of genes in AAA. Methods To determine differences in miRNA levels between AAA (n = 5) and control (n = 5) infrarenal aortic tissues, a microarray study was carried out. Results were adjusted using Benjamini-Hochberg correction (adjusted p Results A microarray study identified eight miRNAs with significantly different expression levels between AAA and controls (adjusted p Conclusions Our genome-wide approach revealed several differentially expressed miRNAs in human AAA tissue suggesting that miRNAs play a role in AAA pathogenesis. |
| Databáze: | OpenAIRE |
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