Evolution of the Na,K- and H,K-ATPase β subunit gene family: Structure of the murine Na,K-ATPase β2 subunit gene
| Autor: | Victor A. Canfield, Andrew W. Shyjan, Robert Levenson |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
RFC5
Molecular Sequence Data Molecular Conformation Regulatory Sequences Nucleic Acid Biology Gamma-aminobutyric acid receptor subunit alpha-1 RFC2 H(+)-K(+)-Exchanging ATPase Mice SCN3A Sequence Homology Nucleic Acid Genetics Animals Gene family Amino Acid Sequence Cloning Molecular GABBR1 Adenosine Triphosphatases Base Sequence Chromosome Mapping Exons Biological Evolution TATA Box Molecular biology Introns Gene Expression Regulation TAF4 TAF2 Sodium-Potassium-Exchanging ATPase |
| Zdroj: | Genomics. 11:435-442 |
| ISSN: | 0888-7543 |
| Popis: | We have cloned and characterized the mouse Na,K-ATPase beta 2 subunit gene (Atp1b2). The gene spans approximately 7 kb and is split into seven exons. The transcription initiation site has been mapped and consensus TATA and putative CAAT sequences have been found at positions -23 and -137, respectively. Discrete structural domains of the beta 2 subunit protein are encoded by separate exons: The intracellular amino-terminal and putative transmembrane domains are encoded by individual exons and the extracellular carboxyl-terminal domain is encoded by five exons. The exon/intron organization of the beta 2 subunit gene closely resembles that of the H,K-ATPase beta subunit gene, suggesting that these two genes evolved from a common evolutionary ancestor. Comparison of the promoter region of the mouse and rat beta 2 subunit genes reveals a remarkably high degree of homology within a 788-nucleotide segment immediately upstream of the transcription start site. This observation suggests that elements that serve to regulate the cell-specific expression of the beta 2 subunit gene are likely to be located within this conserved region. |
| Databáze: | OpenAIRE |
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