Ruscogenin interacts with DPPC and DPPG model membranes and increases the membrane fluidity: FTIR and DSC studies

Autor: Ipek, Sahin, Çağatay, Ceylan, Oguz, Bayraktar
Jazyk: angličtina
Rok vydání: 2023
Předmět:
antioxidant
crystallization
1
2-Dipalmitoylphosphatidylcholine
Membrane Fluidity
Lipid Bilayers
Biophysics
antioxidant activity
concentration (parameter)
IC50
transition temperature
chemistry
Biochemistry
dipalmitoylphosphatidylglycerol
Article
membrane model
Differential scanning calorimetry
enthalpy
Ruscus aculeatus
Drug-membrane interaction
Spectroscopy
Fourier Transform Infrared
Dipalmitoyl phosphatidylcholine
controlled study
phosphatidylglycerol
infrared spectroscopy
Molecular Biology
saponin derivative
Dipalmitoyl phosphatidylglycerol
Ruscogenin
phytosterol
ABTS radical scavenging assay
hydrogen bond
ampholyte
Calorimetry
Differential Scanning
Fourier Analysis
Fourier transform infrared spectroscopy
Phosphatidylglycerols
anion
unclassified drug
lipid bilayer
1
2-dipalmitoylphosphatidylglycerol
Ruscus
phase transition
Ruscus aculeatus extract
liposome
dipalmitoylphosphatidylcholine
hydration
trolox equivalent antioxidant capacity
trolox C
ISSN: 2021-2259
Popis: Ruscogenin, a kind of steroid saponin, has been shown to have significant anti-oxidant, anti-inflammatory, and anti-thrombotic characteristics. Furthermore, it has the potential to be employed as a medicinal medication to treat a variety of acute and chronic disorders. The interaction of a drug molecule with cell membranes can help to elucidate its system-wide protective and therapeutic effects, and it's also important for its pharmacological activity. The molecular mechanism by which ruscogenin affects membrane architecture is still a mystery. Ruscogenin's interaction with zwitterionic dipalmitoyl phosphatidylcholine (DPPC) and anionic dipalmitoyl phosphatidylglycerol (DPPG) multilamellar vesicles (MLVs) was studied utilizing two non-invasive approaches, including: Fourier Transform Infrared (FTIR) spectroscopy and Differential Scanning Calorimetry. Ruscogenin caused considerable alterations in the phase transition profile, order, dynamics and hydration state of head groups and glycerol backbone of DPPC and DPPG MLVs at all concentrations. The DSC results indicated that the presence of ruscogenin decreased the main phase transition temperature (Tm) and enthalpy (?H) values of both membranes and increased half height width of the main transition (?T1/2). The FTIR results demonstrated that all concentrations (1, 3, 6, 9, 15, 24 and 30 mol percent) of ruscogenin disordered the DPPC MLVs both in the gel and liquid crystalline phases while it increased the order of DPPG MLVs in the liquid crystalline phase. Moreover, ruscogenin caused an increase in the dynamics of DPPC and DPPG MLVs in both phases. Additionally, it enhanced the hydration of the head groups of lipids and the surrounding water molecules implying ruscogenin to interact strongly with both zwitterionic and charged model membranes. © 2022 Elsevier Inc.
Ege Üniversitesi: FGA-2021-22592
This work was supported by Ege University Scientific Research Projects Coordination. (Project number is FGA-2021-22592).
This work was supported by Ege University Scientific Research Projects Coordination . (Project number is FGA-2021-22592 ).
Databáze: OpenAIRE
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