Germination and appressorium formation of Pyricularia oryzae Cavara can be inhibited by reduced concentration of Blasin®Flowable with carbon dioxide microbubbles
| Autor: | Ohsato Shuichi, Kobayashi Fumiyuki, Sato Michio, Suehiro Keisuke, Tamaki Masahiko |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Pyricularia Scanning electron microscope Agriculture (General) Plant Science Vacuole 010501 environmental sciences 01 natural sciences Biochemistry S1-972 Conidium chemistry.chemical_compound Food Animals carbon dioxide microbubble 010608 biotechnology Pyricularia oryzae 0105 earth and related environmental sciences Appressorium Ecology biology fungi biology.organism_classification Blasin®Flowable germination chemistry Germination Transmission electron microscopy Carbon dioxide Animal Science and Zoology Agronomy and Crop Science appressorium formation Food Science Nuclear chemistry |
| Zdroj: | Journal of Integrative Agriculture, Vol 17, Iss 9, Pp 2024-2030 (2018) |
| ISSN: | 2095-3119 |
| Popis: | We investigated the possibility to reduce the usage of Blasin®Flowable (BF), a disinfectant inhibiting the germination and appressorium formation of Pyricularia oryzae Cavara conidia, by using carbon dioxide microbubbles (CO2MB). Germination was significantly inhibited by 10 000-fold diluted BF solution containing CO2MB generated by the decompression-type generator compared to CO2 millibubbles (CO2MMB) and CO2MB generated by the gas-water circulating-type generator. Appressorium formation in the 10 000-fold diluted BF solution containing both CO2MBs was less than that in CO2MMB. Scanning electron microscopy showed wrinkles and dents on the surface of conidia treated with 5 000-fold diluted BF solution containing both CO2MBs. Via transmission electron microscopy, we observed the expansion of the vacuole and the intracellular space and bloated or absent lipid granules in the conidia treated with BF solution containing both CO2MBs. Our results show that inhibition of the conidium germination and appressorium formation of P. oryzae Cavara by 10 000-fold diluted BF solution could be achieved by using the decompression-type CO2MB. |
| Databáze: | OpenAIRE |
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