Nerve outgrowth, synaptogenesis and bloelectric activity in fetal rat cerebral cortex tissue cultured in serum-free, chemically defined medium

Autor: H.J. Romijn, P.S. Wolters, A.M.M.C. Habets, M.T. Mud
Rok vydání: 1981
Předmět:
Zdroj: Developmental Brain Research. 2:583-589
ISSN: 0165-3806
DOI: 10.1016/0165-3806(81)90026-2
Popis: Dissociated and non-dissociated cerebral cortex of fetal rat was successfully cultured in a serum-free, chemically defined medium for at least 18 days without any preincubation in serum-supplemented medium. Neurite outgrowth, synapse formation and spontaneous bioelectric activity, in its qualitative and quantitative aspects, were essentially the same as was observed in medium containing 20% heat-inactivated horse serum.
Databáze: OpenAIRE