Nerve outgrowth, synaptogenesis and bloelectric activity in fetal rat cerebral cortex tissue cultured in serum-free, chemically defined medium
Autor: | H.J. Romijn, P.S. Wolters, A.M.M.C. Habets, M.T. Mud |
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Rok vydání: | 1981 |
Předmět: |
Neurite
Synaptogenesis Biology Synaptic Transmission Membrane Potentials Developmental Neuroscience Serum free medicine Animals Synapse formation Cells Cultured Cerebral Cortex Fetus Cell Differentiation Rats Inbred Strains Blood Proteins Culture Media Rats Cell biology Microscopy Electron Chemically defined medium medicine.anatomical_structure Cerebral cortex Synapses Horse serum Neuroscience Developmental Biology |
Zdroj: | Developmental Brain Research. 2:583-589 |
ISSN: | 0165-3806 |
DOI: | 10.1016/0165-3806(81)90026-2 |
Popis: | Dissociated and non-dissociated cerebral cortex of fetal rat was successfully cultured in a serum-free, chemically defined medium for at least 18 days without any preincubation in serum-supplemented medium. Neurite outgrowth, synapse formation and spontaneous bioelectric activity, in its qualitative and quantitative aspects, were essentially the same as was observed in medium containing 20% heat-inactivated horse serum. |
Databáze: | OpenAIRE |
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