HISTOCHEMISTRY OF LACTIC DEHYDROGENASE IN HEART AND PECTORALIS MUSCLES OF RAT
| Autor: | Nobuhisa Baba, Hari M. Sharma |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
Male
Cytoplasm medicine.medical_specialty Hot Temperature Time Factors Cellular respiration Heart Ventricles Sarcoplasm Biology Article Myofibrils Formaldehyde Internal medicine medicine Animals Urea Myocyte Pectoralis Muscle L-Lactate Dehydrogenase Histocytochemistry Tissue Extracts Muscles Myocardium Endoplasmic reticulum Pectoralis major muscle Ground substance Skeletal muscle Rats Inbred Strains Cell Biology Electrophoresis Disc musculoskeletal system Mitochondria Muscle Rats Isoenzymes Microscopy Electron Sarcoplasmic Reticulum Endocrinology medicine.anatomical_structure Biochemistry Tissue Preservation |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.51.3.621 |
| Popis: | Left-ventricular heart muscle and pectoralis major muscle of the rat were studied to determine the intracellular localization of lactic dehydrogenase (LDH) isoenzymes. Fixation of tissue for 2 hr in 2% buffered formaldehyde provided the best preservation of the ultrastructure and enzyme activity. Total LDH activity was found diffusely in the ground substance of the sarcoplasm and in the mitochondria of the heart muscle. In skeletal muscle a strong reaction was noted in the sarcoplasmic reticulum, and moderate activity was seen in the ground substance of the sarcoplasm and in the mitochondria. Differentiation of the isoenzymes of LDH was accomplished by addition of 4 M urea or application of heat. Heart-type isoenzymes were mainly localized in the mitochondria and sarcoplasm, whereas muscle-type isoenzymes were localized mainly in the sarcoplasmic reticulum of the skeletal muscle. It is speculated that the sarcoplasmic reticulum of the skeletal muscle is the site of anaerobic glycolysis and that the sarcoplasm and mitochondria are involved primarily in aerobic metabolism of pyruvate. |
| Databáze: | OpenAIRE |
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