Linkage relationship between the genes for thymidine kinase and galactokinase in different primates
| Autor: | Carlo M. Croce, Thomas A. Tedesco, W.J. Mellman, K.G. Orkwiszewski |
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| Rok vydání: | 1976 |
| Předmět: |
Pan troglodytes
Genetic Linkage Old World monkey Hybrid Cells Thymidine Kinase Chromosomes HAT medium Species Specificity biology.animal Chlorocebus aethiops Genetics Animals Humans Primate biology Phosphotransferases Galactose General Medicine Haplorhini biology.organism_classification Galactokinase Molecular biology Chromosome 17 (human) Genes Thymidine kinase Karyotyping Galactokinase activity African Green Monkey |
| Zdroj: | Somatic cell genetics. 2(1) |
| ISSN: | 0098-0366 |
| Popis: | In this study we investigated the expression of primate galactokinase in somatic cell hybrids between a thymidine kinase-deficient mouse cell line and two different primate cell lines, one of which was derived from African green monkey kidney cells and the other from chimpanzee fibroblasts. All the African green monkey-mouse hybrid clones, selected in HAT medium, expressed monkey galactokinase activity and contained a monkey chromosome similar to a human E-group chromosome. When these clones were backselected in medium containing 5-bromodeoxyuridine, both this chromosome and the monkey galactokinase activity were lost. All the hybrid clones between mouse and chimpanzee cells, which were selected in HAT medium, contained the chimpanzee chromosome 17 and expressed chimpanzee galactokinase activity. These results indicate that the linkage relationship between galactokinase and thymidine kinase has been maintained in 3 divergent primate species--man, chimpanzee, and Old World monkey. |
| Databáze: | OpenAIRE |
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