Biodegradation of 3-chlorobenzoic acid with electron shuttle systems: pathways and molecular identification
| Autor: | Ola A. A. Khalil, Heba A. El-Bialy, Mohamed N Abu El-Naga |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Electrons
medicine.disease_cause Biochemistry Microbiology Redox 03 medical and health sciences Pseudomonas Genetics medicine Molecular Biology Escherichia coli 030304 developmental biology chemistry.chemical_classification Pollutant 0303 health sciences biology 030306 microbiology Pseudomonas putida General Medicine Electron acceptor Biodegradation biology.organism_classification Combinatorial chemistry Chlorobenzoates Biodegradation Environmental chemistry Genes Bacterial Pseudomonas aeruginosa Degradation (geology) Environmental Pollutants Oxidation-Reduction Bacteria |
| Zdroj: | Archives of microbiology. 202(9) |
| ISSN: | 1432-072X |
| Popis: | A synergy of biodegradation and electron shuttle systems is a promising strategy for eliminating pollutants including chlorinated aromatic compounds. The present work studies the degradation products of 3-chlorobenzoic acid by Pseudomonas putida in the presence of an electron shuttle system (ESS) composed of citrate and pyruvate as electron donors and the pollutant as an electron acceptor. Chromatographic results showed different pathways involved in the biodegradation process under the influence of electron shuttle systems. These routes depend on oxidation and reduction reactions for output byproducts to be easily mineralized by the bacterium under investigation. A nucleotide sequence with about 380 bp of a ton B gene was detected in P. putida and it resembles Escherichia coli Ton B. The relatedness tree of the selected gene reveals a high similarity and is comparable to P. aeruginosa (100%) and the highest variation with that of P. citronellolis (21.99%). Accordingly, in the presence of electron shuttle systems, the genes responsible for bacterial influx were activated to ease the biodegradation process. In an application model, the remediated-water samples were handled by two recycling processes using Scenedesmus obliquus and Trigonella foenum-graecum to evaluate the efficiency of this non-conventional treatment. In conclusion, this strategy succeeded in remediating the polluted water with chlorinated aromatic compounds for further applications. |
| Databáze: | OpenAIRE |
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