Low-dose exposure to black carbon significantly increase lung injury of cadmium by promoting cellular apoptosis
Autor: | Pengyu Dang, Jinwei Zhang, Bin Li, Yi Lin, Lingjuan Wang, Fengli Wang, Shanyu Bao, Xiaolong Liu, Fan Wang |
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Rok vydání: | 2021 |
Předmět: |
Health
Toxicology and Mutagenesis Apoptosis Inflammation PM2.5 Pharmacology Lung injury Environmental pollution Cd Cell membrane Mice Soot Carbon black BEAS-2B cells medicine Animals GE1-350 Cytotoxicity Lung Cell damage Chemistry Public Health Environmental and Occupational Health Lung Injury General Medicine medicine.disease Pollution Carbon Environmental sciences medicine.anatomical_structure TD172-193.5 Toxicity medicine.symptom Intracellular Cadmium |
Zdroj: | Ecotoxicology and Environmental Safety, Vol 224, Iss, Pp 112703-(2021) |
ISSN: | 0147-6513 |
DOI: | 10.1016/j.ecoenv.2021.112703 |
Popis: | Particulate matter 2.5 (PM2.5) has adverse biological effects on major living organs in the body, including lungs. The complex composition of PM2.5, including carbon black and heavy metals, cause toxic effects to the lung. Nonetheless, there exists considerable knowledge gaps regarding the impact of carbon black (CB) on environmental health and safety (EHS). Thus far, the synergistic effects of CB have not gained much attention in past decades. Here, we showed that combined exposure of CB and Cadmium (Cd) enhance the cytotoxicity by altering the state of cell membrane. Specially, CB caused cell membrane collapse and increased the permeability of cells, and remarkedly enhanced the metal Cd toxicity. Furthermore, upon pre-treatment sublethal-dose CB, the increased intracellular Cd brought about a significantly amount of lactate dehydrogenase (LDH) and high expression of metallothionein-1 (MT-1) in human lung epithelial cell line (BEAS-2B) cells, and ultimately resulted an increased cellular toxicity. The lung of mice exposed CBs and Cd presented remarkably inflammation than Cd alone. Mechanistic exploration deciphered that CB pre-treatment triggered cell damage via apoptosis due to Cd exposure. Collectively, our findings reveal a novel path for understanding the impact of CB on EHS with its synergistic effects, through which nanomaterials might exert detrimental effects on organisms. |
Databáze: | OpenAIRE |
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