Molecular cytogenetic analysis of formalin-fixed, paraffin-embedded solid tumors by comparative genomic hybridization after universal DNA-amplification
| Autor: | S du Manoir, Christoph Lengauer, H Holtgreve-Grez, Thomas Ried, Michael R. Speicher, B Schoell, Evelin Schröck, Thomas Cremer |
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| Rok vydání: | 1993 |
| Předmět: |
medicine.medical_specialty
Molecular Sequence Data Biology Genome Polymerase Chain Reaction DNA sequencing chemistry.chemical_compound Nucleic acid thermodynamics Formaldehyde Neoplasms Gene duplication Genetics medicine Humans Molecular Biology Genetics (clinical) DNA Primers Chromosome Aberrations Base Sequence Genome Human Histological Techniques Cytogenetics Chromosome Chromosome Mapping Nucleic Acid Hybridization General Medicine DNA Neoplasm Molecular biology chemistry Paraffin Karyotyping DNA Fluorescein-5-isothiocyanate Comparative genomic hybridization |
| Zdroj: | ResearcherID |
| ISSN: | 0964-6906 |
| Popis: | We present a technique which allows the detection and chromosomal localization of DNA sequence copy number changes in solid tumor genomes from frozen sections and paraffin embedded, formalin fixed specimens. Based on comparative genomic hybridization and on universal DNA amplification procedures this technique is possible even if only a few tumor cells are available. We demonstrate the feasibility of this method to visualize complete and partial chromosome gains and losses and gene amplifications in archived solid tumor samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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