Genetically Targeted Adenovirus Vector Directed to CD40-Expressing Cells
| Autor: | Victor Krasnykh, Nikolay Korokhov, Valentina Krendelshchikova, Pierre L. Triozzi, Wayne Aldrich, Galina Mikheeva, Natalya Belousova, Vera Simonenko, Stephen D. Gillies, Papia T. Banerjee, David T. Curiel |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Virus genetics
Adenoviridae Infections Recombinant Fusion Proteins viruses Genetic enhancement Transgene CD40 Ligand Genetic Vectors Immunology Biology Microbiology Virus Cell Line Viral vector Viral Proteins Transduction (genetics) Capsid Transduction Genetic Virology Tumor Cells Cultured Bacteriophage T4 Humans CD40 Antigens Adenoviruses Human Gene Transfer Techniques Gene targeting Dendritic Cells Gene Therapy Insect Science Gene Targeting Receptors Virus Capsid Proteins |
| Zdroj: | Journal of Virology. 77:11367-11377 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | The success of gene therapy depends on the specificity of transgene delivery by therapeutic vectors. The present study describes the use of an adenovirus (Ad) fiber replacement strategy for genetic targeting of the virus to human CD40, which is expressed by a variety of diseased tissues. The tropism of the virus was modified by the incorporation into its capsid of a protein chimera comprising structural domains of three different proteins: the Ad serotype 5 fiber, phage T4 fibritin, and the human CD40 ligand (CD40L). The tumor necrosis factor-like domain of CD40L retains its functional tertiary structure upon incorporation into this chimera and allows the virus to use CD40 as a surrogate receptor for cell entry. The ability of the modified Ad vector to infect CD40-positive dendritic cells and tumor cells with a high efficiency makes this virus a prototype of choice for the derivation of therapeutic vectors for the genetic immunization and targeted destruction of tumors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|