Statistical optimisation of enzymatic detoxification with laccase from Trametes versicolor for spent sulphite liquors using a novel in-situ NMR method
| Autor: | Birgit Kamm, Laura Sparlinek, Viktoria Leitner |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
In situ Paper Magnetic Resonance Spectroscopy Industrial Waste Bioengineering 010501 environmental sciences 01 natural sciences Applied Microbiology and Biotechnology 010608 biotechnology Detoxification Sulfites Dry matter Response surface methodology 0105 earth and related environmental sciences Trametes versicolor Laccase chemistry.chemical_classification Trametes Chromatography Models Statistical biology Chemistry Temperature General Medicine Hydrogen-Ion Concentration biology.organism_classification Enzyme Degradation (geology) Biotechnology |
| Zdroj: | Journal of biotechnology. 284 |
| ISSN: | 1873-4863 |
| Popis: | Within this study the mediated detoxification of spent sulphite liquor via laccase from Trametes versicolor was analysed and optimised using an in-situ NMR-spectroscopy method. The enzymatic degradation kinetic was optimised using the degradation rate of aromatic compounds as indirect parameter. Via response surface methodology the impact of the temperature, the pH and the enzyme concentration was analysed and the conditions were optimised focusing on optimal detoxification. The results of the statistical calculation revealed a valid statistical model for the optimal impact on the aromatic degradation with a temperature of 31 °C, a pH of 6 and a laccase concentrations of 179 U g−1 dry matter of spent sulphite liquor. By using these conditions 88.73% of aromatic compounds could be degraded. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect