Sero-molecular evaluation of Toxoplasma gondii infection among HIV-positive patients
| Autor: | Aleksandra Barac, Reza Pishkarie-Asl, Ehsan Ahmadpour, Hossein Samadi Kafil, Adel Spotin, Hasan Didarlu, Yaghob Azadi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male 0301 basic medicine 030231 tropical medicine Antibodies Protozoan Buffy coat Iran Immunoglobulin G Serology Young Adult 03 medical and health sciences 0302 clinical medicine Seroepidemiologic Studies Humans Medicine Seroprevalence Prospective Studies AIDS-Related Opportunistic Infections biology Reverse Transcriptase Polymerase Chain Reaction business.industry Public Health Environmental and Occupational Health Toxoplasma gondii General Medicine Middle Aged biology.organism_classification medicine.disease Toxoplasmosis 3. Good health Cross-Sectional Studies 030104 developmental biology Infectious Diseases Immunoglobulin M Luminescent Measurements Immunology biology.protein Female Parasitology Antibody business Toxoplasma |
| Zdroj: | Transactions of The Royal Society of Tropical Medicine and Hygiene. 113:771-775 |
| ISSN: | 1878-3503 0035-9203 |
| DOI: | 10.1093/trstmh/trz082 |
| Popis: | Background Toxoplasmosis is one of the most common comorbidities in HIV-positive patients with CD4+ T lymphocytes below 200 cells/μl. Early diagnosis and treatment of toxoplasmosis reduces the mortality rate in HIV-positive people. The aim of this study was to estimate the seroprevalence of Toxoplasma gondii infection in HIV-positive patients in northwest Iran using serological and molecular methods. Methods This prospective cross-sectional study included 124 HIV-positive outpatients and was conducted from January to May 2016. Anti-T. gondii IgM and IgG antibodies were detected from sera samples by chemiluminescence, while buffy coat samples were analyzed by RT-PCR for DNA detection. Patients’ socioepidemiological data were collected. Results Using chemiluminescence, 47/124 samples (37.9%) were positive for anti-Toxoplasma IgG antibodies, 2/124 samples (1.62%) were positive for IgM antibodies while 2/124 samples (1.62%) contained both IgM and IgG. There were no IgM-positive or IgG-negative patients. RT-PCR revealed four (3.22%) positive samples. On the basis of the results, a statistically significant relationship was found between anti-Toxoplasma IgG antibody seropositivity and residence (p=0.012). Conclusions The study showed a relatively low seroprevalence of anti-T. gondii IgG and IgM antibodies in HIV-positive patients in northwest Iran, while the prevalence was much higher in other regions of Iran. However, regular screening for T. gondii antibodies and early initiation of therapy are very important to decrease the mortality rate in HIV-positive patients. |
| Databáze: | OpenAIRE |
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