BβArg448Lys polymorphism is associated with altered fibrin clot structure and fibrinolysis in type 2 diabetes
| Autor: | Jackie F. Price, Kristina F. Standeven, Mark W. J. Strachan, Katie A. Greenhalgh, Paul D. Baxter, Robert F. Storey, Robert A. S. Ariëns, Ramzi A. Ajjan, Peter J. Grant, S. H. Alzahrani |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Protein Conformation medicine.medical_treatment Type 2 diabetes 030204 cardiovascular system & hematology Fibrinogen DISEASE MELLITUS Diabetes mellitus 0302 clinical medicine Risk Factors Antithrombotic genetic polymorphism INSULIN-RESISTANCE biology Fibrinolysis Hematology Middle Aged Thrombosis Phenotype Female Blood Coagulation Tests medicine.drug medicine.medical_specialty Genotype BETA COAGULATION 030209 endocrinology & metabolism Risk Assessment Fibrin Structure-Activity Relationship 03 medical and health sciences HYPERGLYCEMIA Internal medicine medicine Humans Genetic Association Studies thrombosis Aged Polymorphism Genetic Vascular disease business.industry medicine.disease Endocrinology Diabetes Mellitus Type 2 Scotland MYOCARDIAL-INFARCTION Immunology Microscopy Electron Scanning biology.protein vascular diseases fibrinogen business GLYCATION |
| Zdroj: | Greenhalgh, KA, Strachan, M W, Alzahrani, S H, Baxter, P D, Standeven, KF, Storey, R F, Ariens, R A S, Grant, P J, Price, J & Ajjan, R A 2017, ' BβArg448Lys polymorphism is associated with altered fibrin clot structure and fibrinolysis in type 2 diabetes ', Thrombosis and Haemostasis, vol. 117, no. 2, pp. 295-302 . https://doi.org/10.1160/TH16-07-0554 |
| ISSN: | 2567-689X 0340-6245 |
| Popis: | SummaryBoth type 2 diabetes (T2DM) and Bß448Lys variant of fibrinogen are associated with dense fibrin clots, impaired fibrinolysis and increased cardiovascular risk. It was our objective to investigate whether BßArg448Lys adds to vascular risk by modulating fibrin network structure and/or fibrinolysis in diabetes. The primary aim was to study effects of BßArg448Lys on fibrin network characteristics in T2DM. Secondary aims investigated interactions between gender and BßArg448Lys substitution in relation to fibrin clot properties and vascular disease. Genotyping for BßArg448Lys and dynamic clot studies were carried out on 822 T2DM patients enrolled in the Edinburgh Type 2 Diabetes Study. Turbidimetric assays of individual plasma samples analysed fibrin clot characteristics with additional experiments conducted on clots made from purified fibrinogen, further examined by confocal and electron microscopy. Plasma clot lysis time in Bß448Lys was longer than Bß448Arg variant (mean ± SD; 763 ± 322 and 719 ± 351 seconds [s], respectively; p |
| Databáze: | OpenAIRE |
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