Contribution of in Vivo Experimental Challenges to Understanding Flat Oyster Ostrea edulis Resistance to Bonamia ostreae
| Autor: | Sophie Lerond, Isabelle Arzul, Benjamin Morga, Jean-Pierre Joly, Tristan Renault, Celine Garcia, Sylvie Lapegue, Estelle Harrang, Bruno Chollet, Nicole Faury |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Oyster Hemocytes Time Factors Ostrea edulis Haplosporida Phagocytosis Immunology lcsh:QR1-502 Bonamia ostreae Gene Expression Context (language use) Microbiology lcsh:Microbiology Host-Parasite Interactions resistance 03 medical and health sciences Immunity biology.animal Animals 14. Life underwater Shellfish Original Research Disease Resistance Protozoan Infections biology protozoan Ecology fungi apoptosis food and beverages equipment and supplies biology.organism_classification Ostreidae 030104 developmental biology Infectious Diseases experimental infection haemocytes gene expression bacteria Reactive Oxygen Species |
| Zdroj: | Frontiers in Cellular and Infection Microbiology Frontiers in Cellular and Infection Microbiology, Vol 7 (2017) Frontiers In Cellular And Infection Microbiology (2235-2988) (Frontiers Media Sa), 2017-10, Vol. 7, N. 433, P. 1-13 |
| ISSN: | 2235-2988 |
| Popis: | Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality outbreaks in European flat oyster stocks in Europe. As eradication and treatment are not possible, the control of the disease mainly relies on transfer restriction. Moreover, selection has been applied to produce resistant flat oyster families, which present better survival and lower prevalence than non-selected oysters. In order to better understand the mechanisms involved in resistance to bonamiosis, cellular and molecular responses of 2 oyster groups (selected oysters and wild-type oysters) were analyzed in the context of experimental injection and cohabitation infections. Cellular responses including non-specific esterases detection, ROS production and phagocytosis activity were analyzed by flow cytometry. Four genes homologous to those shown to be involved in immunity were selected (Inhibitor of apotosis OeIAP, Fas ligand OeFas-ligand, Oe-SOD, and OeEc-SOD) and monitored by quantitative reverse-transcription PCR (qRT-PCR). Infected oysters showed higher phagocytosis activity than controls. Infected selected oyster show a lower phagocytosis activity which might be a protection against the parasite infection. The expression of OeIAP and OeFas-ligand gene was significantly increased in selected oysters at 5 days post-injection. OeIAP gene expression appeared to be significantly increased in wild-type oysters at 8 days post-injection. Our results suggest that resistance to bonamiosis partly relies on the ability of the oysters to modulate apoptosis. |
| Databáze: | OpenAIRE |
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